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Purified Mouse Anti-SRP54
Purified Mouse Anti-SRP54

Western blot analysis of SRP54 on Jurkat cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-SRP54.

Purified Mouse Anti-SRP54

Immunofluorescent staining of Human Endothelial cells with anti-SRP54.

Western blot analysis of SRP54 on Jurkat cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-SRP54.

Immunofluorescent staining of Human Endothelial cells with anti-SRP54.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Frog (Tested in Development)
Mouse IgG1
Human SRP54 aa. 262-476
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
54 kDa
250 µg/ml
AB_398254
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610940 Rev. 1
Antibody Details
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30/SRP54

Ribosomes that exist freely in the cytosol or those attached to the ER are  intrinsically the same in their translational properties. ER-bound ribosomes are responsible for the production of secretory proteins and integral ER, Golgi, lysosomal, and plasma membrane spanning proteins. Such proteins contain signal sequences that direct their synthesis to the ER membrane. As the nascent  polypeptide emerges from the ribosome, a signal recognition particle (SRP) binds to the signal sequence and serves to couple the ribosome to the protein-translocating machinery in the ER membrane. Although the SRP is a 325 kDa ribonucleoprotein, its 54 kDa subunit (SRP54) mediates interaction with, and targeting of, the nascent protein to the ER. Via its C-terminal M-domain, SRP54 associates with the nascent protein and inhibits its elongation. This complex binds  to the SRP receptor on the ER, the ribosome is delivered to the translocation machinery, SRP is released, and elongation resumes. Targeting and insertion are tightly coupled to a GTPase cycle that involves SRP54 and SRP receptor. Although the mechanisms are unclear, release of SRP from the ER-bound complex requires GTP hydrolysis.  

610940 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610940 Rev.1
Citations & References
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Development References (2)

  1. Rapiejko PJ, Gilmore R. Empty site forms of the SRP54 and SR alpha GTPases mediate targeting of ribosome-nascent chain complexes to the endoplasmic reticulum. Cell. 1997; 89(5):703-713. (Biology). View Reference
  2. Traianedes K, Findlay DM, Martin TJ, Gillespie MT. Modulation of the signal recognition particle 54-kDa subunit (SRP54) in rat preosteoblasts by the extracellular matrix. J Biol Chem. 1995; 270(36):20891-20894. (Biology). View Reference
610940 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.