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Purified Mouse Anti-SNX2
Purified Mouse Anti-SNX2

Western blot analysis of SNX2 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1: 2000 dilution of the mouse anti- SNX2 antibody.

Purified Mouse Anti-SNX2

Immunofluorescence staining of ES-2 cells (Human ovary clear cell carcinoma; ATCC CRL-1978).

Western blot analysis of SNX2 on a human endothelial cell lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1: 2000 dilution of the mouse anti- SNX2 antibody.

Immunofluorescence staining of ES-2 cells (Human ovary clear cell carcinoma; ATCC CRL-1978).

Product Details
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BD Transduction Laboratories™
Sorting Nexin-2
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human SNX2 aa. 15-137
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
72 kDa
250 µg/ml
AB_398834
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Antibody Details
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13/SNX2

Biological processes such as transmembrane signaling and receptor mediated endocytosis revolve around the function of cell surface receptors. A network of molecular machinery directs the intracellular trafficking of receptors during their biosynthesis and mediates signaling downstream of receptors. The sorting nexins (SNX1, SNX1A, SNX2, SNX3, and SNX4) are a family of intracellular proteins that are thought to direct the sorting of receptor proteins. The SNX proteins contain a conserved 100 amino acid region termed the phox homology (PX) domain and are part of a family of hydrophilic proteins which includes S. cerevisiae proteins that function in protein sorting. SNX1, SNX2, and SNX4 associate predominantly with membranes and bind transmembrane receptors such as those for EGF, PDGF, and insulin. SNX1 directs the EGF receptor to the lysosomes for degradation. SNX2 forms homomeric complexes and heteromeric complexes with SNX1, SNX1A, and SNX4. These complexes are thought to be necessary for efficient protein sorting. Thus, SNX2 and other sorting nexins are thought to play important roles in the specificity of protein trafficking to and from the plasma membrane.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (2)

  1. Haft CR, de la Luz Sierra M, Barr VA, Haft DH, Taylor SI. Identification of a family of sorting nexin molecules and characterization of their association with receptors. Mol Cell Biol. 1998; 18(12):7278-7287. (Biology). View Reference
  2. Kurten RC, Cadena DL, Gill GN. Enhanced degradation of EGF receptors by a sorting nexin, SNX1. Science. 1996; 272(5264):1008-1010. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.