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Purified Mouse Anti-LSF
Purified Mouse Anti-LSF

Western blot analysis of LSF on a HCT-8 cell lysate (Human colorectal adenocarcinoma; ATCC CCL-244). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1 2000 dilution of the mouse anti- LSF antibody.

Purified Mouse Anti-LSF

Immunofluorescence staining of MDCK cells (Canine kidney; ATCC CCL-34).

Western blot analysis of LSF on a HCT-8 cell lysate (Human colorectal adenocarcinoma; ATCC CCL-244). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1 2000 dilution of the mouse anti- LSF antibody.

Immunofluorescence staining of MDCK cells (Canine kidney; ATCC CCL-34).

Product Details
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BD Transduction Laboratories™
αCP2; LBP-1c
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human LSF aa. 205-414
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
63 kDa
250 µg/ml
AB_398137
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610818 Rev. 1
Antibody Details
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14/LSF

LSF (αCP2 or LBP-1c) is a transcription factor originally identified by its binding to, and induction of, the SV40 late promoter. LSF displays some similarity to the Elf-1/NTF transcription factors of Drosophila. It is primarily found in its dimeric form in solution, however it is the tetrameric form that recognizes a center-to-center 10 bp repeat. LSF-1D (LBP-1d) is the product of an alternative splicing event, resulting in a deletion of a 51 amino acid segment that corresponds with the DNA-binding region of LSF. LSF-1D is found in the cytoplasm, not in the nucleus, and can interact with LSF to form a heterotetramer that can interfere with LSF DNA binding. In mitogen-stimulated human peripheral T cells, LSF is  phosphorylated on amino acid 291, which increases its DNA binding activity. Thus, LSF-mediated gene transcription is regulated by its phosphorylation and/or its interaction with LSF-1D during cell growth.

This antibody is routinely tested by western blot analysis.  Other applications were tested in BD Biosciences Pharmingen during antibody development only or reported in the literature.

610818 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610818 Rev.1
Citations & References
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Development References (2)

  1. Shirra MK, Zhu Q, Huang HC, Pallas D, Hansen U. One exon of the human LSF gene includes conserved regions involved in novel DNA-binding and dimerization motifs. Mol Biol Cell. 1994; 14(8):5076-5087. (Biology). View Reference
  2. Volker JL, Rameh LE, Zhu Q, DeCaprio J, Hansen U. Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity. Genes Dev. 1997; 11(46):1435-1446. (Biology). View Reference
610818 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.