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Purified Mouse Anti-Hsp90
Purified Mouse Anti-Hsp90

Western blot analysis of Hsp90 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Hsp90 antibody.

Purified Mouse Anti-Hsp90

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).

Western blot analysis of Hsp90 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Hsp90 antibody.

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).

Product Details
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BD Transduction Laboratories™
Heat Shock Protein-90
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human Hsp90 aa. 586-732
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
90 kDa
250 µg/ml
AB_397799
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610418 Rev. 1
Antibody Details
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68/Hsp90

The 90 kDa heat shock protein (Hsp90), a highly conserved stress-induced protein, is abundantly expressed in most tissues under nonstress conditions and is required for eukaryotic cell viability. Hsp90 interacts with a number of signaling molecules, other heat shock proteins, and cytoskeletal proteins. However, the function of Hsp90 in these complexes remains unclear. It is thought that Hsp90 plays a common role in a number of diverse signaling systems due to its physical association with v-Src family tyrosine kinases, steroid hormone receptors, the Raf serine/threonine kinase, the basic-helix-loop-helix dioxin receptor, and the βγ subunits of G proteins.

610418 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610418 Rev.1
Citations & References
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Development References (5)

  1. Brouet A, Sonveaux P, Dessy C, Balligand JL, Feron O. Hsp90 ensures the transition from the early Ca2+-dependent to the late phosphorylation-dependent activation of the endothelial nitric-oxide synthase in vascular endothelial growth factor-exposed endothelial cells. J Biol Chem. 2001; 276(35):32663-32669. (Biology: Immunoprecipitation, Western blot). View Reference
  2. Miyamoto A, Nakayama K, Imaki H. Increased proliferation of B cells and auto-immunity in mice lacking protein kinase Cdelta. Nature. 2002; 416(6883):865-869. (Biology: Western blot). View Reference
  3. Murata T, Sato K, Hori M, Ozaki H, Karaki H. Decreased endothelial nitric-oxide synthase (eNOS) activity resulting from abnormal interaction between eNOS and its regulatory proteins in hypoxia-induced pulmonary hypertension. J Biol Chem. 2002; 277(46):44085-44092. (Biology: Western blot). View Reference
  4. Pritchard KA Jr, Ackerman AW. Heat shock protein 90 mediates the balance of nitric oxide and superoxide anion from endothelial nitric-oxide synthase. J Biol Chem. 2001; 276(21):17621-17624. (Biology: Western blot). View Reference
  5. Rebbe NF, Ware J, Bertina RM, Modrich P, Stafford DW. Nucleotide sequence of a cDNA for a member of the human 90-kDa heat-shock protein family. Gene. 1987; 53(2-3):235-245. (Biology). View Reference
View All (5) View Less
610418 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.