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Purified Mouse Anti-FKBP12
Purified Mouse Anti-FKBP12

Western blot analysis of FKBP12 on a SW-13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-FKBP12 antibody.

Purified Mouse Anti-FKBP12

Immunofluorescence staining of HS 766T cells (Human pancreatic carcinoma; ATCC HTB-134).

Western blot analysis of FKBP12 on a SW-13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-FKBP12 antibody.

Immunofluorescence staining of HS 766T cells (Human pancreatic carcinoma; ATCC HTB-134).

Product Details
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BD Transduction Laboratories™
FK506 Binding Protein 1A
Human (QC Testing), Mouse, Dog, Chicken (Tested in Development)
Mouse IgG1
Human FKBP12 aa. 1-108
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
14 kDa
250 µg/ml
AB_2102850
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610808 Rev. 1
Antibody Details
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8/FKBP12

Transforming growth factor-β binds to the TGFβ family of heteromeric serine/threonine transmembrane receptors (type I and type II).  Following binding of TGFβ, the type II receptor (TβR-II) phosphorylates the type I receptor (TβR-I) which, in turn, conveys the signal.  Since TβR-I and TβR-II can interact without the stimulation of TGFβ, leading to unwanted activation, a regulatory mechanism exists.  In a yeast genetic screen, immunophilin FKBP12 was associated with the type I receptor. Studies including co-immunoprecipiation, deletion, and point mutations confirmed this interaction.  FKBP12 inhibits TβR-II mediated phosphorylation of TβR-I, inhibiting activation.  FKBP12 binds via its rapamycin/Leu-Pro binding pocket to the Leu-Pro sequence adjacent to the phosphorylation site of TβR-I.  This interaction is blocked by the addition of  macrolides, rapamycin, and FK506.  Furthermore, mutations in the binding sites of FKBP12 and TβR-I abrogates the binding and results in activation of the receptor without the addition of TGFβ.  Thus, FKBP12 is a regulatory protein for TβR-I and TβR-II-mediated signaling.

610808 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610808 Rev.1
Citations & References
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Development References (2)

  1. Chen YG, Liu F, Massague J. Mechanism of TGFbeta receptor inhibition by FKBP12. EMBO J. 1997; 16(13):3866-3876. (Biology). View Reference
  2. Wang T, Donahoe PK, Zervos AS. Specific interaction of type I receptors of the TGF-beta family with the immunophilin FKBP-12. Science. 1994; 265(5172):674-676. (Biology). View Reference
610808 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.