Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Dynamin is a nerve terminal phosphoprotein with intrinsic GTPase activity which plays an important role in endocytosis. This GTPase activity is stimulated in vitro by microtubules, SH3 domain-containing proteins, phospholipids, and Protein Kinase C (PKC)-mediated phosphorylation. There are at least two distinct dynamin genes in mammals, which show 79% identity, encoding proteins Dynamin I and Dynamin II. Dynamin I is expressed almost exclusively in the central nervous system while Dynamin II expression is ubiquitous. Depolarization stimulates synaptic vesicle recycling and Dynamin I is subsequently dephosphorylated. This rapid dephosphorylation is mediated by calcineurin which acts as a switch for depolarization-initiated synaptic vesicle endocytosis. The C-terminus of dynamin contains two clusters of proline-rich SH3 domain binding proline motifs which interact with known SH3 domain proteins during tyrosine kinase receptor activation. It is reported that clone 41 detects both dynamin I and dynamin II.
Development References (5)
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Hayes S, Chawla A, Corvera S. TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2. J Cell Biol. 2002; 158(7):1239-1249. (Clone-specific: Immunofluorescence). View Reference
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Kalthoff C, Groos S, Kohl R, Mahrhold S, Ungewickell EJ. Clint: a novel clathrin-binding ENTH-domain protein at the Golgi. Mol Biol Cell. 2002; 13(11):4060-4073. (Clone-specific). View Reference
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Ochoa GC, Slepnev VI, Neff L, et al. A functional link between dynamin and the actin cytoskeleton at podosomes. J Cell Biol. 2000; 150(2):377-389. (Clone-specific: Western blot). View Reference
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Scaife R, Gout I, Waterfield MD, Margolis RL. Growth factor-induced binding of dynamin to signal transduction proteins involves sorting to distinct and separate proline-rich dynamin sequences. EMBO J. 1994; 13(11):2574-2582. (Biology). View Reference
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Schmidlin F, Dery O, DeFea KO, et al. Dynamin and Rab5a-dependent trafficking and signaling of the neurokinin 1 receptor. J Biol Chem. 2001; 276(27):25427-25437. (Clone-specific: Immunofluorescence, Western blot). View Reference
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