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Purified Mouse Anti-Casein Kinase Iε
Purified Mouse Anti-Casein Kinase Iε

Western blot analysis of Casein Kinase Iε on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Casein Kinase Iε antibody.

Purified Mouse Anti-Casein Kinase Iε

Immunofluorescence staining of human fibroblast cells stained with the mouse anti-Casein Kinase Iε antibody.

 

Western blot analysis of Casein Kinase Iε on a human endothelial cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-Casein Kinase Iε antibody.

Immunofluorescence staining of human fibroblast cells stained with the mouse anti-Casein Kinase Iε antibody.

 

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Chicken, Dog (Tested in Development)
Mouse IgG1
Human Casein Kinase Iε aa. 248-414
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
47 kDa
250 µg/ml
AB_397820
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610445 Rev. 1
Antibody Details
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1/Casein Kinase Iε

Casein Kinase Iε (CKIε) is a product of a family of proteins involved in the regulation of various cytoplasmic and nuclear processes including DNA replication and repair. CKIε is a 47.3 kDa serine/threonine kinase that plays a role similar to previously described CKI isoforms. It phosphorylates known CKI substrates, including a CKI-specific peptide. In addition, it is inhibited by CKI-7, an inhibitor of other CKI isoforms. CKIε contains a core kinase domain of 285 amino acids which is 53-98% identical to other isoforms and is most similiar in structure to CKIδ. Since various human and yeast CKI proteins exhibit both sequence and functional similarities, CKIε may be involved in mammalian DNA metabolism.

610445 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610445 Rev.1
Citations & References
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Development References (4)

  1. Akashi M, Tsuchiya Y, Yoshino T, Nishida E. Control of intracellular dynamics of mammalian period proteins by casein kinase I epsilon (CKIepsilon) and CKIdelta in cultured cells. Mol Cell Biol. 2002; 22(6):1693-1703. (Biology: Western blot). View Reference
  2. Fish KJ, Cegielska A, Getman ME, Landes GM, Virshup DM. Isolation and characterization of human casein kinase I epsilon (CKI), a novel member of the CKI gene family. J Biol Chem. 1995; 270(25):14875-14883. (Biology). View Reference
  3. Kishida M, Hino Si, Michiue T, et al. Synergistic activation of the Wnt signaling pathway by Dvl and casein kinase Iepsilon. J Biol Chem. 2001; 276(35):33147-33155. (Biology: Western blot). View Reference
  4. Peters JM, McKay RM, McKay JP, Graff JM. Casein kinase I transduces Wnt signals. Nature. 1999; 401(6751):345-350. (Biology: Western blot). View Reference
View All (4) View Less
610445 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.