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Purified Mouse Anti-Cadherin-5
Purified Mouse Anti-Cadherin-5
Western blot analysis of Cadherin-5 on human endothelial cell lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Cadherin-5 antibody.
Purified Mouse Anti-Cadherin-5
Immunofluorescent staining of Human Fibroblasts with anti-Cadherin-5 antibody.
Western blot analysis of Cadherin-5 on human endothelial cell lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Cadherin-5 antibody.
Immunofluorescent staining of Human Fibroblasts with anti-Cadherin-5 antibody.
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human Cadherin 5 aa. 26-194
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
130 kDa
250 µg/ml
AB_2276073
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610251 Rev. 1
Antibody Details
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75/Cadherin-5

Cadherins are a family of transmembrane glycoproteins involved in the Ca2+- dependent cell-cell adhesion that occurs in many tissues. These proteins are similar in their domain structure (45-74% amino acid conservation), Ca2+ and protease-sensitivity, and molecular weight. Cadherin-5 (VE-Cadherin or CD144) is one of a number of cadherins (cadherin-4 through -11) whose cDNAs were isolated from rat brain and retina. These cadherins have a cytoplasmic domain that is highly conserved relative to previously identified cadherins, indicating that this domain is essential for cell adhesion activity. This function is mediated by cadherin interaction with cytoskeletal proteins. However, Cadherin-5's cytoplasmic domain has the lowest degree of homology with the other cadherins. Cadherin-5 is expressed in brain and various other tissues, including umbilical cord vein endothelial cells. A new type of adhering junction has been identified in certain vascular endothelial cells. These junctions are known as "complexus adherens" and are morphologically and compositionally distinct from desmosomes and zonula adherens junctions. The complexus adherens of endothelial cells lack desmosomal cadherins as well as E-Cadherin. However, these cells are rich in Cadherin-5 which colocalizes with desmoplakin and γ-Catenin (plakoglobin).

610251 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610251 Rev.1
Citations & References
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View product citations for antibody "610251" on CiteAb

Development References (5)

  1. Corada M, Liao F, Lindgren M. Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability. Blood. 2001; 97(6):1679-1684. (Clone-specific: Immunofluorescence, Western blot). View Reference
  2. Corada M, Zanetta L, Orsenigo F. A monoclonal antibody to vascular endothelial-cadherin inhibits tumor angiogenesis without side effects on endothelial permeability. Blood. 2002; 100(3):905-911. (Clone-specific: Flow cytometry, Western blot). View Reference
  3. Rahimi N, Kazlauskas A. A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells. Mol Biol Cell. 1999; 10:3401-3407. (Clone-specific: Functional assay). View Reference
  4. Schmelz M, Franke WW. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. J Cell Biol. 1993; 61(2):274-289. (Biology). View Reference
  5. Suzuki S, Sano K, Tanihara H. Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue. Cell Regul. 1991; 2(4):261-270. (Biology). View Reference
View All (5) View Less
610251 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.