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Purified Mouse Anti-AP50
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Mouse AP50/µ2 aa. 110-230
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
50 kDa
250 µg/ml
AB_398873
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/support/resources/cell_biology/index.jsp

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611350 Rev. 1
Antibody Details
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31/AP50

Sorting of integral membrane proteins is mediated vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large adaptin proteins (γ, α, δ, ε, and β1, β2, β3, β4 respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, µ4 respectively) and one small chain ( σ1, σ2, σ3, σ4 respectively). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2, µ2 (AP50) interacts with integral membrane proteins via binding to tyrosine-based signals with the canonical motif YXXΦ. In addition, AP50/µ2 is required for both the assembly and the proton transport activity of vacuolar (H+)-ATPases in clathrin coated vesicles. Thus, AP50/µ2 may be involved in targeting integral membrane proteins that are sorted based on tyrosine-based signals and involved in assembly of functional ion channels associated with clathrin coated vesicles.

611350 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611350 Rev.1
Citations & References
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Development References (2)

  1. Ohno H, Stewart J, Fournier MC et al. Interaction of tyrosine-based sorting signals with clathrin-associated proteins. Science. 1995; 269(5232):1872-1875. (Biology). View Reference
  2. Vecchi M, Polo S, Poupon V, van de Loo JW, Benmerah A, Di Fiore PP. Nucleocytoplasmic shuttling of endocytic proteins. J Cell Biol. 2001; 153(7):1511-1517. (Clone-specific: Immunofluorescence). View Reference
611350 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.