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      Purified Mouse Anti-Annexin I
      Purified Mouse Anti-Annexin I
      Western blot analysis of Annexin I on a human endothelial cell lysate.  Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Annexin I antibody.
      Purified Mouse Anti-Annexin I
      Immunohistochemical staining of a rabbit cerebrum section.
      Purified Mouse Anti-Annexin I Purified Mouse Anti-Annexin I
      Western blot analysis of Annexin I on a human endothelial cell lysate.  Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-Annexin I antibody.
      Immunohistochemical staining of a rabbit cerebrum section.
      Product Details
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      BD Transduction Laboratories™
      Lipocortin-1; Calpactin II
      Human (QC Testing), Dog (Tested in Development)
      Mouse IgG1
      Cow Annexin I aa. 1-346
      Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
      38 kDa
      250 µg/ml
      AB_397477
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Recommended Assay Procedures

      Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      610067 Rev. 1
      Antibody Details
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      29/Annexin I

      Annexin I, also known as lipocortin-1 or calpactin II, is part of a calcium-dependent phospholipid-binding protein family. Members share a  common core domain, but each has a unique N-terminal tail that imparts its functional specificity. It is believed that phosphorlyation of this region in annexin I regulates its functions. Annexin I has been implicated in regulating phospholipid vesicle aggregation, mediating inflammatory response, and inhibiting the activity of phospholipase A2. Additionally, it has been found that the 10 kDa protein S100C influences annexin I's activity by binding to the N-terminal 13 residues of annexin in a manner similar to that of the annexin II/p11 complex.

      610067 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610067 Rev.1
      Citations & References
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      View product citations for antibody "610067" on CiteAb

      Development References (4)

      1. Chasserot-Golaz S, Vitale N, Sagot I, et al. Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells. J Cell Biol. 1996; 133(6):1217-1236. (Biology). View Reference
      2. Graham ME, Gerke V, Burgoyne RD. Modification of annexin II expression in PC12 cell lines does not affect Ca(2+)-dependent exocytosis. Mol Biol Cell. 1997; 8(3):431-442. (Biology). View Reference
      3. Hansen MD, Ehrlich JS, Nelson WJ. Molecular mechanism for orienting membrane and actin dynamics to nascent cell-cell contacts in epithelial cells. J Cell Biol. 2002; 277(47):45371-45376. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
      4. Regnouf F, Sagot I, Delouche B, et al. "In vitro" phosphorylation of annexin 2 heterotetramer by protein kinase C. Comparative properties of the unphosphorylated and phosphorylated annexin 2 on the aggregation and fusion of chromaffin granule membranes. J Biol Chem. 1995; 270(45):27143-27150. (Biology). View Reference
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      610067 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.