Recombinant Mouse C5a protein is useful as a standard in a sandwich ELISA that measures mouse C5a protein levels in plasma or serum samples. Purified I52-1486 Anti-Mouse C5a (Cat. No. 558027) as capture antibody can be paired with the biotinylated I52-278 Anti-Mouse C5a (Cat. No. 558028) as the detection antibody, using recombinant mouse C5a protein as standard. The purified I52-1486 antibody should be titrated between 1-4 µg/mL to determine the optimal ELISA plate-coating concentration. To obtain linear standard curves, doubling dilutions of recombinant mouse C5a protein ranging from 78 pg/mL to 5 ng/mL are recommended for inclusion in each ELISA plate. Addition of FUT-175 (Futhan, Cat. No. 552035) to plasma samples at the time of sample collection provides additional protection from ex-vivo activation, and therefore ensures more accurate measurements that reflect the circulating levels of complement activation products. This ELISA antibody pair shows no cross-reactivity with the following: recombinant mouse C3a, native human, and rat C5a.
The amount of protein provided is listed on the vial label, record the batch number and the concentration of the reconstituted protein here and on the vial of the single-use aliquots for future reference:________ ng/mL.
Warning: Recombinant Mouse C5a (Cat. No. 622597) contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).
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