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RB744 Mouse Anti-Human CD130 (gp130)
RB744 Mouse Anti-Human CD130 (gp130)
Multiparameter flow cytometric analysis using BD OptiBuild™ RB744 Mouse Anti-Human CD130 (gp130) antibody (Cat. No. 756845; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 570519; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
RB744 Mouse Anti-Human CD130 (gp130)
Multiparameter flow cytometric analysis using BD OptiBuild™ RB744 Mouse Anti-Human CD130 (gp130) antibody (Cat. No. 756845; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 570519; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
Multiparameter flow cytometric analysis using BD OptiBuild™ RB744 Mouse Anti-Human CD130 (gp130) antibody (Cat. No. 756845; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 570519; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
Multiparameter flow cytometric analysis using BD OptiBuild™ RB744 Mouse Anti-Human CD130 (gp130) antibody (Cat. No. 756845; Right Plot) on Human peripheral blood, with corresponding IgG Isotype Control (Cat. No. 570519; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
Product Details
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BD OptiBuild™
IL6ST; IL6RB; IL-6Rβ; Interleukin-6 Receptor beta; gp130
Human (Tested in Development)
Mouse IgG1, κ
Purified Human CD130
Flow cytometry (Qualified)
0.2 mg/ml
V C041
3572
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  10. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  11. For U.S. patents that may apply, see bd.com/patents.
756845 Rev. 0
Antibody Details
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AM64

The AM64 monoclonal antibody specifically binds to CD130 which is also known as gp130 (Membrane glycoprotein 130), IL6ST (Interleukin-6 signal transducer), and IL6RB/IL-6Rβ (Interleukin-6 Receptor beta). CD130 is a 130 kDa type 1 membrane glycoprotein that belongs to the hemopoietic cytokine receptor superfamily. CD130 serves as a common signaling receptor for several cytokine receptor complexes including those that mediate the biological effects of IL-6, IL-11, IL-27, Cardiotrophin-1 (CT-1), Ciliary neurotrophic factor (CNTF), Leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130 is widely expressed on a variety of cell types including T cells, activated B cells, monocytes, plasma cells, and endothelia. The AM64 antibody can be used in flow cytometry, immunoprecipitation, western blot, and immunohistochemistry on frozen sections.

756845 Rev. 0
Format Details
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RB744
The BD Horizon RealBlue™ 744 (RB744) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 746-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB744 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), we recommend using an optical filter centered near 750-nm (e.g., a 750/60-nm bandpass filter).
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RB744
Blue 488 nm
498 nm
746 nm
756845 Rev.0
Citations & References
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Development References (4)

  1. Hibi M, Murakami M, Saito M, Hirano T, Taga T, Kishimoto T. Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 1990; 63(6):1149-1157. (Immunogen: Blocking, Flow cytometry, Immunoprecipitation). View Reference
  2. Pietzko D, Zohlnhöfer D, Graeve L, et al. The hepatic interleukin-6 receptor. Studies on its structure and regulation by phorbol 12-myristate 13-acetate-dexamethasone. J Biol Chem. 1993; 268(6):4250-4258. (Clone-specific: Immunoprecipitation). View Reference
  3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
  4. Zola H, Fusco M, Flego L, Donohoe PJ, Macardle PJ. Expression of the Cytokine Receptor Panel antibodies: high-sensitivity immunofluorescence studies on unstimulated cells. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1939-1941.
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756845 Rev. 0

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.