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RB545 Mouse Anti-Human TCR Vα24
Product Details
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BD OptiBuild™
TCR Va24; TCR Valpha24; TCR Vα24; TCRAV24S1; TCRV alpha 24; TRAV24
Human (Tested in Development)
Mouse BALB/c IgG1, κ
Human T-cell clone
Flow cytometry (Qualified)
0.2 mg/ml
28659
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  3. For U.S. patents that may apply, see bd.com/patents.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. An isotype control should be used at the same concentration as the antibody of interest.
  10. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
758082 Rev. 1
Antibody Details
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C15

The C15 monoclonal antibody specifically recognizes the variable alpha 24 region of the α subunit of the human αβ T cell receptor for antigen (TCR Vα24). TCR Vα24 combines with joining region 18 (Jα18) to form Vα24-Jα18 (Arden nomenclature, or TRAV10-TRAJ18 in the IMGT nomenclature). TCR Vα24 can pair with different TCR Vβ region containing TCR β chains. The paired expression of TCR Vα24 with semivariant TCR Vβ11 identifies CD3+TCR αβ+ positive thymocytes and from ~ 0.01-0.92%  of peripheral blood CD3+ TCR αβ+ T cells known as invariant NKT (iNKT) cells or Type I NKT cells. iNKT cells can be divided into three subpopulations which are either CD4+, CD8+, or CD4-CD8- double negative. Due to their innate-like responsiveness, tissue resident iNKT cells are capable of rapid effector responses to microbial or self-lipid antigens presented by the cell surface MHC class Ib molecule, CD1d.  iNKT cells recognize and respond to α-galactosylceramide (α-GalCer) and various self-lipids such as sphingolipids and diacylglycerols and microbial lipids presented by CD1d. iNKT cells can produce high levels of cytokines, including IL-4 and IFN-γ, and act as cytotoxic T cells against CD1d+ target cells following stimulation. The C15 antibody is useful for multiparameter analyses designed to study the nature of TCR Vα24-positive T cells and T cell clones. When combined with other TCR V region-specific antibodies, C15 is helpful for characterizing TCR Vα repertoires expressed by T cell populations collected from blood, tissues or other sources in health and disease models including inflammation, infectious diseases, autoimmunity, and tumors. The C15 antibody reportedly stimulates the proliferation of TCR Vα24-positive T cells.

758082 Rev. 1
Format Details
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RB545
The BD Horizon RealBlue™ 545 (RB545) fluorochrome is part of the BD family of blue (488-nm) laser dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 496-nm and an emission maximum (Em Max) at 545-nm. Driven by BD innovation, RB545 can be used on spectral cytometers and is designed to be excited by the blue (488-nm) laser with minimal excitation by the 561-nm yellow-green laser. RB545 has minimal spillover into yellow-green detectors and a brightness level similar to FITC.  Given its unique emission max, on a spectral instrument RB545 can be used simultaneously with FITC and PE to provide an additional color off of the blue laser. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
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RB545
Blue 488 nm
496 nm
545 nm
758082 Rev.1
Citations & References
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Development References (10)

  1. Arden B, Clark SP, Kabelitz D, Mak TW. Human T-cell receptor variable gene segment families.. Immunogenetics. 1995; 42(6):455-500. (Biology). View Reference
  2. Dellabona P, Padovan E, Casorati G, Brockhaus M, Lanzavecchia A. An invariant V alpha 24-J alpha Q/V beta 11 T cell receptor is expressed in all individuals by clonally expanded CD4-8- T cells.. J Exp Med. 1994; 180(3):1171-6. (Immunogen: Flow cytometry, Fluorescence activated cell sorting). View Reference
  3. Elliott JI. Selection of dual Valpha T cells.. Eur J Immunol. 1998; 28(7):2115-23. (Clone-specific: Flow cytometry). View Reference
  4. Godfrey DI, Uldrich AP, McCluskey J, Rossjohn J, Moody DB. The burgeoning family of unconventional T cells.. Nat Immunol. 2015; 16(11):1114-23. (Biology). View Reference
  5. Kent SC, Hafler DA, Strominger JL, Wilson SB. Noncanonical Valpha24JalphaQ T cells with conservative alpha chain CDR3 region amino acid substitutions are restricted by CD1d.. Hum Immunol. 1999; 60(11):1080-9. (Clone-specific: Flow cytometry). View Reference
  6. Montoya CJ, Pollard D, Martinson J, et al. Characterization of human invariant natural killer T subsets in health and disease using a novel invariant natural killer T cell-clonotypic monoclonal antibody, 6B11.. Immunology. 2007; 122(1):1-14. (Clone-specific: Flow cytometry). View Reference
  7. Padovan E, Casorati G, Dellabona P, Meyer S, Brockhaus M, Lanzavecchia A. Expression of two T cell receptor alpha chains: dual receptor T cells.. Science. 1993; 262(5132):422-4. (Immunogen: Flow cytometry, Fluorescence activated cell sorting). View Reference
  8. Prell C, Konstantopoulos N, Heinzelmann B, et al. Frequency of Valpha24+CD161+ natural killer T cells and invariant TCRAV24-AJ18 transcripts in atopic and non-atopic individuals.. Immunobiology. 2003; 208(4):367-80. (Clone-specific: Flow cytometry). View Reference
  9. Scaviner D, Lefranc MP. The human T cell receptor alpha variable (TRAV) genes.. Exp Clin Immunogenet. 2000; 17(2):83-96. (Biology). View Reference
  10. Vyth-Dreese FA, Sein J, van de Kasteele W, et al. Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma.. Clin Exp Immunol. 2010; 162(3):447-59. (Clone-specific: Flow cytometry). View Reference
View All (10) View Less
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.