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![RB545 Mouse Anti-Human CD45RA](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/756xxx/7565xx/756515_base/756515_Image1.png)
![RB545 Mouse Anti-Human CD45RA](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/756xxx/7565xx/756515_base/756515_Image1.png)
![RB545 Mouse Anti-Human CD45RA](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/756xxx/7565xx/756515_base/756515_Image2.png)
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![RB545 Mouse Anti-Human CD45RA](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/756xxx/7565xx/756515_base/756515_Image1.png)
Multiparameter flow cytometric analysis using BD OptiBuild™ RB545 Mouse Anti-Human CD45RA antibody (Cat. No. 756515; Right Plot) on Human peripheral blood lymphocytes, with corresponding IgG Isotype Control (Cat. No. 569292; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed using FlowJo™ v10.8 software.
![RB545 Mouse Anti-Human CD45RA](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/756xxx/7565xx/756515_base/756515_Image2.png)
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Green Cap - Blue Label (Transparent).png)
![RB545 Mouse Anti-Human CD45RA](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/756xxx/7565xx/756515_base/756515_Image1.png)
BD OptiBuild™ RB545 Mouse Anti-Human CD45RA
![RB545 Mouse Anti-Human CD45RA](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/756xxx/7565xx/756515_base/756515_Image2.png)
BD OptiBuild™ RB545 Mouse Anti-Human CD45RA
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Green Cap - Blue Label (Transparent).png)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Companion Products
![sampleImage/](/content/dam/bdb/products/global/reagents/cell-preparation-separation-reagents/blood-lysis/staining-and-cell-preparation/554xxx/5546xx/554656_base/554656_01.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/global/reagents/cell-preparation-separation-reagents/blood-lysis/staining-and-cell-preparation/554xxx/5546xx/554657_base/554657_01.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/clinical-diagnostics/buffers-and-supporting-reagents-ivd-ce-ivd/349xxx/3492xx/349202_base/349202_01.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/564xxx/5642xx/564219_base/FcBlock_de.png?imwidth=320)
The HI100 monoclonal antibody specifically binds to the 220 kDa isoform of the human leukocyte common antigen, CD45RA. CD45RA is expressed on approximately 40-50% of peripheral CD4+ T cells, 50% of peripheral CD8+ T cells and on a portion of B cells and monocytes. The CD45RA antigen is expressed by naïve and activated T cells. CD45RA-specific antibodies are useful for the study of the suppressor/inducer subpopulation of CD4+ lymphocytes.
![altImg](/content/dam/bdb/products/vialimages/packaging-images/formatimages/RB545_Excited_Dye.jpg)
No Citations are available for this Product
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.