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R718 Rat Anti-Mouse CD206 (Mannose Receptor)
R718 Rat Anti-Mouse CD206 (Mannose Receptor)
Analysis of CD206 (Mannose Receptor) Expression by Thioglycolate-elicited Peritoneal Exudate Cells (Thio PECs)    Surface CD206 (Mannose Receptor) Expression - Panel 1: Thio PECs were stained with FITC Rat Anti-Mouse CD107b antibody (Cat. No. 553323/​562061) and with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; Top Plot​) or BD Horizon™ R718 Rat Anti-Mouse CD206 (Mannose Receptor) antibody (Cat. No. 569000/569001; Bottom Plot) at 1.0 μg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 (Mannose Receptor) [or Ig Isotype control staining] versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells.    Surface and Intracellular CD206 (Mannose Receptor) Expression - Panel 2: Thio PECs were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723) and stained as previously described at 1.0 µg/test. The bivariate pseudocolor density plots showing the correlated expression of CD206 (Mannose Receptor) [or Ig Isotype control staining] versus CD107b were derived from gated events with the forward and side light-scatter characteristics of intact cells.    Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific and were generated in separate experiments.
Analysis of CD206 (Mannose Receptor) Expression by Thioglycolate-elicited Peritoneal Exudate Cells (Thio PECs)    Surface CD206 (Mannose Receptor) Expression - Panel 1: Thio PECs were stained with FITC Rat Anti-Mouse CD107b antibody (Cat. No. 553323/​562061) and with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; Top Plot​) or BD Horizon™ R718 Rat Anti-Mouse CD206 (Mannose Receptor) antibody (Cat. No. 569000/569001; Bottom Plot) at 1.0 μg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD206 (Mannose Receptor) [or Ig Isotype control staining] versus CD107b was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells.    Surface and Intracellular CD206 (Mannose Receptor) Expression - Panel 2: Thio PECs were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723) and stained as previously described at 1.0 µg/test. The bivariate pseudocolor density plots showing the correlated expression of CD206 (Mannose Receptor) [or Ig Isotype control staining] versus CD107b were derived from gated events with the forward and side light-scatter characteristics of intact cells.    Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific and were generated in separate experiments.
Product Details
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BD Horizon™
CD206; MMR; Mrc1; macrophage mannose receptor 1
Mouse (QC Testing)
Rat WI, also known as Wistar (outbred) IgG2a, κ
Mouse CD206 Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
17533
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  7. Alexa Fluor™ is a trademark of Life Technologies Corporation.
  8. For U.S. patents that may apply, see bd.com/patents.
Antibody Details
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Y17-505

The Y17-505 monoclonal antibody specifically binds to CD206 which is also known as the Macrophage mannose receptor (MMR, MR) or Mannose receptor, C type 1 (Mrc1). CD206 is a type I transmembrane glycoprotein of approximately 175 kDa that belongs to the C-type lectin superfamily. It is expressed at the cell surface and intracellularly by macrophages, Langerhans cells, dendritic cells, and endothelial cells within hepatic and lymphoid tissues. This pattern recognition receptor binds to endogenous and microbial glycoconjugates containing mannose, fucose, or N-acetylglucosamine through its C-type lectin-like carbohydrate recognition domains (CRD). CD206 also contains a cysteine-rich domain that enables binding to sulfated carbohydrate antigens. This receptor enables macrophages and other specialized cells to maintain tissue homeostasis as well as to internalize microbes or their components by phagocytosis or endocytosis. CD206 thus plays important roles in mediating innate immunity, eg, enabling phagocytosis, as well as in processing and presenting antigens for the generation and expression of adaptive immunity. Moreover, CD206 has been associated with leucocyte homing and cancer cell metastasis.

Format Details
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R718
The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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R718
Red 627-640 nm
695 nm
718 nm
Citations & References
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Development References (6)

  1. Akbarshahi H, Menzel M, Posaric Bauden M, Rosendahl A, Andersson R. Enrichment of murine CD68+ CCR2+ and CD68+ CD206+ lung macrophages in acute pancreatitis-associated acute lung injury. PLoS ONE. 2012; 7(10):e42654. (Biology). View Reference
  2. Burgdorf S, Lukacs-Kornek V, Kurts C. The mannose receptor mediates uptake of soluble but not of cell-associated antigen for cross-presentation. J Immunol. 2006; 176(11):6770-6776. (Biology). View Reference
  3. Marttila-Ichihara F, Turja R, Miiluniemi M, et al. Macrophage mannose receptor on lymphatics controls cell trafficking. Blood. 2008; 112(1):64-72. (Biology). View Reference
  4. McKenzie EJ, Taylor PR, Stillion RJ, et al. J Immunol. 2007; 178(8):4975-4983. (Biology). View Reference
  5. Rybalko V, Hsieh PL, Merscham-Banda M, Suggs LJ, Farrar RP. The Development of Macrophage-Mediated Cell Therapy to Improve Skeletal Muscle Function after Injury.. PLoS One. 2015; 10(12):e0145550. (Biology). View Reference
  6. Zamze S, Martinez-Pomares L, Jones H, et al. Recognition of bacterial capsular polysaccharides and lipopolysaccharides by the macrophage mannose receptor. J Biol Chem. 2002; 277(44):41613-41623. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.