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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Companion Products
The P-3E10 monoclonal antibody specifically recognizes CD298, the β3 subunit of the Na+/K+ ATPase (Sodium/potassium-transporting ATPase subunit beta-3). CD298 is a ~45-55 kDa single-pass, type II membrane protein that is encoded by ATP1B3 (ATPase Na+/K+ transporting subunit beta 3) which belongs to the P-type ATPases superfamily. CD298 is widely expressed on lymphocytes, monocytes, granulocytes, platelets, and on other hematopoietic and nonhematopoietic cells and cell lines. The Na+/K+ ATPase is an integral membrane protein complex with enzymatic activity that mediates the active transport and exchange of sodium and potassium ions across the plasma membrane. This complex is composed of α and β subunits. The α subunit is a 10-membrane-spanning, catalytic protein that contains binding sites for Na +, K + and ATP. The α subunits are associated with the smaller, regulatory glycoprotein β subunits. Upon ATP hydrolysis, the Na+/K+ ATPase transports Na+ ions out of the cell in exchange for K+ ions that are transported in. This establishes a transmembrane electrochemical gradient that is essential for osmoregulation and for the transport of various nutrients and other molecules by cells. The P-3E10 antibody can reportedly inhibit the activation and proliferation of T cells and B cells.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.
Development References (5)
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Bouwer AL, Saunderson SC, Caldwell FJ, et al. NK cells are required for dendritic cell-based immunotherapy at the time of tumor challenge.. J Immunol. 2014; 192(5):2514-21. (Clone-specific: Flow cytometry). View Reference
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P. A. Gorer, S. Lyman, G. D. Snell. Studies on the Genetic and Antigenic Basis of Tumour Transplantation. Linkage between a Histocompatibility Gene and 'Fused' in Mice. Proc R Soc Lond B Biol Sci. 1948; 135(881):499-505. (Biology).
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Springer TA. Cell-surface differentiation in the mouse. Characterization of "jumping" and "lineage" antigens using xenogeneic rat monoclonal antibodies. In: Kennett RH, McKearn TJ, Bechtol KB, ed. Monoclonal antibodies. Hybridomas: A new dimension in biological analyses. New York and London: Plenum Press; 1980:185-217.
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Stallcup KC, Springer TA, Mescher MF. Characterization of an anti-H-2 monoclonal antibody and its use in large-scale antigen purification. J Immunol. 1981; 127(30):923-930. (Immunogen: Flow cytometry, Immunoaffinity chromatography, Immunoprecipitation, Radioimmunoassay). View Reference
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Watanabe Y, Kuribayashi K, Miyatake S, et al. Exogenous expression of mouse interferon gamma cDNA in mouse neuroblastoma C1300 cells results in reduced tumorigenicity by augmented anti-tumor immunity.. Proc Natl Acad Sci USA. 1989; 86(23):9456-60. (Clone-specific: Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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