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PE Mouse Anti-Mouse TCR Vγ7
PE Mouse Anti-Mouse TCR Vγ7
Flow cytometric analysis of TCR Vγ7 expression on Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with BD OptiBuild™ R718 Hamster Anti-Mouse γδ T-Cell Receptor (Cat. No. 751919) and FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061/561827) antibodies and with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 553457; Left Plot) or PE Mouse Anti-Mouse TCR Vγ7 antibody (Cat. No. 569154/569155; Right Plot) at 0.5 μg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vγ7 (or Ig Isotype control staining) versus TCR γδ was derived from gated events with the forward and side light- scatter characteristics of viable (DAPI-negative) CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of TCR Vγ7 expression on Mouse splenic T cells. C57BL/6 Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142]. The cells were then stained with BD OptiBuild™ R718 Hamster Anti-Mouse γδ T-Cell Receptor (Cat. No. 751919) and FITC Hamster Anti-Mouse CD3e (Cat. No. 553062/553061/561827) antibodies and with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 553457; Left Plot) or PE Mouse Anti-Mouse TCR Vγ7 antibody (Cat. No. 569154/569155; Right Plot) at 0.5 μg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of TCR Vγ7 (or Ig Isotype control staining) versus TCR γδ was derived from gated events with the forward and side light- scatter characteristics of viable (DAPI-negative) CD3e-positive splenic T lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
T Cell Receptor Vγ7; TCR Vg7; TCR Vgamma7; Tcrg-V7; Trgv7
Mouse (QC Testing)
Mouse IgG2a, κ
Mouse γδ T Cell Hybridoma Cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
21641
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
569154 Rev. 2
Antibody Details
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F2.67

The F2.67 monoclonal antibody specifically recognizes the variable gamma 7 region of the γ subunit of the mouse γδ T cell receptor for antigen, TCR Vγ7 (using the Heilig and Tonegawa nomenclature for mouse TCR γ and δ chains). TCR Vγ7 is encoded by the Trgv7 (T cell receptor gamma, variable 7) gene element. TCR Vγ7 is expressed by a subset of TCR γδ+ thymocytes in the late fetal and adult thymus and by γδ T cells in peripheral lymphoid tissues. TCR Vγ7+ γδ T cells predominate in intestinal epithelial tissue which contains a large proportion of these γδ T cells derived from extrathymic generation. Proteins encoded by Btnl1 (butyrophilin-like 1) and Btnl6 (butyrophilin-like 6) are expressed by intestinal epithelial cells. These butyrophilin-like molecules can reportedly shape the TCR-dependent development and function of TCR Vg7+ γδ T cells within the gut. TCR Vγ7+ γδ T cells help maintain the integrity of the intestinal mucosa guarding against cellular stress or damage caused by inflammation, transformation, or infection. The F2.67 antibody is useful for TCR Vγ7+ thymocyte and γδ T cell separations and analyzing TCR Vγ repertoires expressed by thymocytes, peripheral T cells, and T cell hybridomas in developmental and other experimental model systems.

569154 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
569154 Rev.2
Citations & References
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Development References (12)

  1. Cossarizza A, Chang HD, Radbruch A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).. Eur J Immunol. 2019; 49(10):1457-1973. (Clone-specific: Flow cytometry). View Reference
  2. Dalton JE, Cruickshank SM, Egan CE, et al. Intraepithelial gammadelta+ lymphocytes maintain the integrity of intestinal epithelial tight junctions in response to infection.. Gastroenterology. 2006; 131(3):818-29. (Clone-specific: Cell separation, Flow cytometry). View Reference
  3. Garman RD, Doherty PJ, Raulet DH. Diversity, rearrangement, and expression of murine T cell gamma genes.. Cell. 1986; 45(5):733-42. (Biology). View Reference
  4. Hatano S, Tun X, Noguchi N, et al. Development of a new monoclonal antibody specific to mouse Vγ6 chain. Life Sci Alliance. 2019; 2:3. (Clone-specific: Flow cytometry). View Reference
  5. Heilig JS, Tonegawa S. Diversity of murine gamma genes and expression in fetal and adult T lymphocytes.. Nature. 322(6082):836-40. (Biology: Flow cytometry). View Reference
  6. Kashani E, Föhse L, Raha S, et al. A clonotypic Vγ4Jγ1/Vδ5Dδ2Jδ1 innate γδ T-cell population restricted to the CCR6⁺CD27⁻ subset.. Nat Commun. 2015; 6:6477. (Clone-specific: Flow cytometry). View Reference
  7. Melandri D, Zlatareva I, Chaleil RAG, et al. The γδTCR combines innate immunity with adaptive immunity by utilizing spatially distinct regions for agonist selection and antigen responsiveness.. Nat Immunol. 2018; 19(12):1352-1365. (Clone-specific: Flow cytometry). View Reference
  8. Pereira P, Boucontet L. Rates of recombination and chain pair biases greatly influence the primary gammadelta TCR repertoire in the thymus of adult mice.. J Immunol. 2004; 173(5):3261-70. (Clone-specific: Flow cytometry). View Reference
  9. Pereira P, Gerber D, Huang SY, Tonegawa S. Ontogenic development and tissue distribution of V gamma 1-expressing gamma/delta T lymphocytes in normal mice.. J Exp Med. 1995; 182(6):1921-30. (Biology). View Reference
  10. Pereira P, Hermitte V, Lembezat MP, Boucontet L, Azuara V, Grigoriadou K. Developmentally regulated and lineage-specific rearrangement of T cell receptor Valpha/delta gene segments. Eur J Immunol. 2000; 30(7):1988-1997. (Immunogen: Flow cytometry). View Reference
  11. Sell S, Dietz M, Schneider A, Holtappels R, Mach M, Winkler TH. Control of murine cytomegalovirus infection by γδ T cells.. PLoS Pathog. 2015; 11(2):e1004481. (Clone-specific: Flow cytometry). View Reference
  12. Zeng W, O'Brien RL, Born WK, Huang Y. Characterization of Mouse γδ T Cell Subsets in the Setting of Type-2 Immunity.. Methods Mol Biol. 2018; 1799:135-151. (Clone-specific: Flow cytometry). View Reference
View All (12) View Less
569154 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.