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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Companion Products
The Ba13 monoclonal antibody specifically recognizes Cell surface glycoprotein CD200 receptor 3 (CD200R3) which is also known as CD200 cell surface glycoprotein receptor-like b (CD200RLb), CD200 receptor-like 3, and OX-2 Receptor 3. CD200R3 is a single-pass type I transmembrane glycoprotein that is encoded by Cd200r3 (CD200 receptor 3) which belongs to the immunoglobulin superfamily (IgSF). It is expressed on basophils and mast cells that are involved in protective immune responses against parasitic infections and in the development of allergic reactions. Cell surface CD200R3 presents as a disulfide-linked dimeric CD200R-like receptor. The cytoplasmic tail region of CD200R3 contains a positively charged lysine residue which allows this receptor to associate and signal through adaptor proteins such as DAP10, DAP12, CD3z, or FcRγ. CD200R3 functions as an activating receptor to regulate IgE-independent immune responses.
Development References (3)
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Kojima T, Obata K, Mukai K, et al. Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner.. J Immunol. 2007; 179(10):7093-100. (Biology). View Reference
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Nei Y, Obata-Ninomiya K, Tsutsui H, et al. GATA-1 regulates the generation and function of basophils.. Proc Natl Acad Sci U S A. 2013; 110(46):18620-5. (Clone-specific: Flow cytometry). View Reference
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Pellefigues C, Mehta P, Prout MS, et al. The Basoph8 Mice Enable an Unbiased Detection and a Conditional Depletion of Basophils.. Front Immunol. 2019; 10:2143. (Clone-specific: Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.