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BV711 Rat Anti-Mouse Siglec-E
Product Details
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BD OptiBuild™
Siglec12; Siglece; mSiglec-E; myeloid inhibitory siglec; siglec-12; siglec-E
Mouse (Tested in Development)
Rat IgG2b
Mouse Siglec-E Recombinant Protein
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872620
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV711 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 711 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  10. Cy is a trademark of GE Healthcare.
  11. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
748159 Rev. 3
Antibody Details
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750620

The 750620 monoclonal antibody specifically recognizes Sialic acid binding Ig-like lectin E (Siglec-E).  Siglec-E is a type I transmembrane glycoprotein that is encoded by Siglece which belongs to the CD33-related subfamily of Siglecs within the immunoglobulin gene superfamily (IgSF). The extracellular region of Siglec-E contains an N-terminal V-type Ig domain followed by a C2 type 1 and C2 type 2 domain whereas the intracellular region has an immunoreceptor tyrosine-based inhibitory motif (ITIM) followed by a SLAM-like motif. Siglec-E mediates sialic-acid dependent binding to cells. Siglec-E is expressed on neutrophils and monocytes and can function as an inhibitory receptor which regulates the recruitment and effector responses of these cells.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm.  BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).  Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors.  However, the spillover can be corrected through compensation as with any other dye combination.

748159 Rev. 3
Format Details
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BV711
The BD Horizon Brilliant Violet™ 711 (BV711) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 713-nm. BV711, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 710-nm (e.g., a 712/20-nm bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV711
Violet 405 nm
407 nm
713 nm
748159 Rev.3
Citations & References
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View product citations for antibody "748159" on CiteAb

Development References (2)

  1. Siddiqui S, Schwarz F, Springer S, et al. Studies on the Detection, Expression, Glycosylation, Dimerization, and Ligand Binding Properties of Mouse Siglec-E. J Biol Chem. 2017; 292(3):1029-1037. (Biology). View Reference
  2. Wu Y, Ren D, Chen GY. Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis.. J Immunol. 2016; 197(8):3336-3347. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
748159 Rev. 3

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.