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APC Mouse Anti-Human Integrin α1 (CD49a)

BD Pharmingen™ APC Mouse Anti-Human Integrin α1 (CD49a)

Clone TS2/7 (also known as TS2/7)

(RUO)
APC Mouse Anti-Human Integrin α1 (CD49a)
Multiparameter flow cytometric analysis of Integrin α1 (CD49a) expression on Human leucocyte populations. Human whole blood was stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Plot) or APC Mouse Anti-Human Integrin α1 (CD49a) antibody (Cat. No. 569036/569037; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of Integrin α1 (CD49a) [or Ig Isotype control staining] versus side light-scatter signals (SSC-A) was derived from gated events with the forward and side-light scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
APC Mouse Anti-Human Integrin α1 (CD49a)
Flow cytometric analysis of Integrin α1 (CD49a) expression on HeLa cells. Cells from the Human HeLa (Adenocarcinoma, ATCC® CCL-2™) cell line were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; dashed line histogram) or APC Mouse Anti-Human Integrin α1 (CD49a) antibody (Cat. No. 569036/569037; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing Integrin α1 (CD49a) expression (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
Multiparameter flow cytometric analysis of Integrin α1 (CD49a) expression on Human leucocyte populations. Human whole blood was stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; Left Plot) or APC Mouse Anti-Human Integrin α1 (CD49a) antibody (Cat. No. 569036/569037; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of Integrin α1 (CD49a) [or Ig Isotype control staining] versus side light-scatter signals (SSC-A) was derived from gated events with the forward and side-light scatter characteristics of viable leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
Flow cytometric analysis of Integrin α1 (CD49a) expression on HeLa cells. Cells from the Human HeLa (Adenocarcinoma, ATCC® CCL-2™) cell line were stained with either APC Mouse IgG1, κ Isotype Control (Cat. No. 554681; dashed line histogram) or APC Mouse Anti-Human Integrin α1 (CD49a) antibody (Cat. No. 569036/569037; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing Integrin α1 (CD49a) expression (or Ig Isotype control staining) was derived from gated events with the forward and side-light scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
ITGA1; CD49a; VLA-1; VLA1; integrin alpha-1
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human HLA-DR CTL line
Flow cytometry (Routinely Tested)
5 µl/test
3672
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Antibody Details
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TS2/7

The TS2/7 monoclonal antibody specifically recognizes Integrin alpha 1 (Integrin α1) which is also known as CD49a (CD49 antigen-like family member A) and Very late antigen 1α subunit (VLA-1). Integrin α1 (CD49a) is an ~200 kDa single pass type I transmembrane glycoprotein that is encoded by ITGA1 (Integrin subunit alpha 1) which belongs to the Integrin alpha chain family. Integrin α1 (CD49a) associates with the integrin β1 chain (CD29) to form the Integrin α1β1 heterodimer (also known as CD49a/CD29 or the VLA-1 complex) that serves as a receptor for collagen and laminin-1. It is expressed on activated T cells, monocytes, neuronal cells, and smooth muscle cells. Integrin α1 (CD49a) reportedly plays a role in leucocyte migration into tissues and can costimulate T cell proliferation and cytokine production. It is also involved in cellular attachment during the development of both the central and peripheral nervous systems.

Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
Citations & References
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Development References (5)

  1. Hemler ME, Sanchez-Madrid F, Flotte TJ, et al. Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines.. J Immunol. 1984; 132(6):3011-8. (Clone-specific: Flow cytometry, Immunohistochemistry). View Reference
  2. Marquardt N, Kekäläinen E, Chen P, et al. Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells.. Nat Commun. 2019; 10(1):3841. (Clone-specific: Flow cytometry). View Reference
  3. Melssen MM, Olson W, Wages NA, et al. Formation and phenotypic characterization of CD49a, CD49b and CD103 expressing CD8 T cell populations in human metastatic melanoma.. Oncoimmunology. 7(10):e1490855. (Clone-specific: Flow cytometry). View Reference
  4. Sanchez-Madrid F, Krensky AM, Ware CF, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.. Proc Natl Acad Sci U S A. 1982; 79(23):7489-93. (Immunogen: Western blot). View Reference
  5. Zola H. CD49a. In: Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007:122.
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.