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Alexa Fluor™ 647 Rabbit Anti-Human LDH
Alexa Fluor™ 647 Rabbit Anti-Human LDH
Flow cytometric analysis of LDH expression in HeLa cells.  Cells from the Human HeLa (Cervical adenocarcinoma, ATCC® CCL-2™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with Alexa Fluor™ 647 Rabbit IgG Isotype Control (Cat. No. 569345; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human LDH antibody (Cat. No. 570529; solid line histogram) at 0.03 µg/test. The fluorescence histogram showing LDH expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of LDH expression in HeLa cells.  Cells from the Human HeLa (Cervical adenocarcinoma, ATCC® CCL-2™) cell line were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were then stained with Alexa Fluor™ 647 Rabbit IgG Isotype Control (Cat. No. 569345; dashed line histogram) or Alexa Fluor™ 647 Rabbit Anti-Human LDH antibody (Cat. No. 570529; solid line histogram) at 0.03 µg/test. The fluorescence histogram showing LDH expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
LDH heart subunit; LDH muscle subunit; LDH testis subunit; LDH-H; LDH-M; LDH-X; LDHA; LDHB; LDHC
Human (QC Testing)
Rabbit IgG
Not Reported
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
3939, 3945, 3948
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
  10. This rabbit monoclonal (RabMabTM) product is, in part, provided under an intellectual property license from Abcam. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product for buyer’s internal research use only (whether the buyer is an academic or for-profit entity) and buyer shall not be permitted to reverse engineer or otherwise perform any compositional, structural, functional or other analyses directed to learning the methodology, formulae, sequences, process, make-up or production of this product (or any portion thereof). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. If you, as the buyer, do not agree to these conditions, please leave the product unopened and return the contents of this package to BD Biosciences, 10975 Torreyana Road, San Diego, CA 92121. For information on purchasing a license to this product for purposes other than research, contact Abcam at partnerships@abcam.com.
  11. For U.S. patents that may apply, see bd.com/patents.
570529 Rev. 1
Antibody Details
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EP1563Y

The EP1563Y recombinant monoclonal antibody binds to Lactate dehydrogenase (LDH). This tetrameric cytoplasmatic enzyme belongs to the 2-hydroxy acid oxidoreductase family whose subunits are encoded by LDHA, LDHB, LDHC, and LDHD genes. EP1563Y specifically recognizes LDH-A, LDH-B and LDH-C. LDH-A and LDH-B can form 5 tetrameric isoenzymes: LDH-1 (highly expressed in heart and erythrocytes), LDH-2 (reticuloendothelial system, erythrocytes), LDH-3 (lungs), LDH-4 (kidneys) and LDH-5 (liver, skeletal muscle, brain). LDH-C is specifically expressed in the testis. LDH has a key role in the anaerobic metabolic pathway as it catalyzes the synthesis of lactate and pyruvate in a reversible reaction, serving as an important check point of gluconeogenesis and DNA metabolism. LDH is overexpressed by cells in certain diseases such as cancer and can be released into the bloodstream due to tissue damage caused by injury or disease.

570529 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
570529 Rev.1
Citations & References
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Development References (4)

  1. Adeva M, González-Lucán M, Seco M, Donapetry C. Enzymes involved in l-lactate metabolism in humans.. Mitochondrion. 2013; 13(6):615-29. (Biology). View Reference
  2. Khan AA, Allemailem KS, Alhumaydhi FA, Gowder SJT, Rahmani AH. The Biochemical and Clinical Perspectives of Lactate Dehydrogenase: An Enzyme of Active Metabolism.. Endocr Metab Immune Disord Drug Targets. 2020; 20(6):855-868. (Biology). View Reference
  3. Mishra D, Banerjee D. Lactate Dehydrogenases as Metabolic Links between Tumor and Stroma in the Tumor Microenvironment.. Cancers (Basel). 2019; 11(6):750. (Biology). View Reference
  4. Shi Y, Pinto BM.. Human lactate dehydrogenase a inhibitors: a molecular dynamics investigation.. PLoS One. 2014; 9(1):e86365. (Biology). View Reference
View All (4) View Less
570529 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.