Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Denmark (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      Purified Mouse Anti-TNP
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Mouse BALB/c IgG1, κ
      TNP-keyhole limpet hemocyanin
      ELISA (Routinely Tested), Isotype control (Tested During Development)
      0.5 mg/ml
      AB_395222
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      This immunoglobulin is useful for quantitation of TNP by the sandwich ELISA. For the sandwich TNP ELISA, mAb 107.3 is optimal for capture with anti-TNP 49.2 mAb (Cat. no. 554055) for detection.

      Caution: Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservation agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format for in vitro and in vivo use.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      554054 Rev. 6
      Antibody Details
      Down Arrow Up Arrow
      107.3

      The 107.3 clone has an unknown specificity. Trinitrophenal (TNP), the immunogen, is a hapten not expressed on human or mouse cells. In the absence of specific binding, this antibody may bind non-specifically to Fc receptors. The immunoglobulin from clone 107.3 was selected as an isotype control following screening for low background on a variety of mouse and human tissues.

      This antibody is routinely tested by ELISA analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

      554054 Rev. 6
      Format Details
      Down Arrow Up Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      554054 Rev.6

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback