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Anti-Human Control γ1 PE/CD45 PerCP
Product Details
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BD FastImmune™
Human
Controls
RUO (GMP)
Phosphate buffered saline with BSA and 0.1% sodium azide.


Description

The Mouse γ1 (IgG1) antibody, clone X40, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.

The CD45 antibody, clone 2D1, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

The Mouse γ1 (IgG1) antibody recognizes a keyhole limpet hemocyanin (KLH) antigen not expressed on human cells.

The CD45 antibody recognizes a 180- to 220-kilodalton (kDa) human leucocyte antigen that is a member of the leucocyte common antigen (LCA) family.


The Mouse γ1 (IgG1) antibody, clone X40, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with KLH.

The CD45 antibody, clone 2D1, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood mononuclear cells (PBMCs).

The Mouse γ1 (IgG1) antibody recognizes a keyhole limpet hemocyanin (KLH) antigen not expressed on human cells.

The CD45 antibody recognizes a 180- to 220-kilodalton (kDa) human leucocyte antigen that is a member of the leucocyte common antigen (LCA) family.

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340416 Rev. 1
Components
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Description Clone Isotype EntrezGene ID
Mouse IgG1 Isotype Control PE L293 IgG1, κ N/A
CD45 PerCP C8Mab-1 IgG2b, λ N/A
340416 Rev. 1
Citations & References
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Development References (6)

  1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  2. Clinical and Laboratory Standards Institute. 2005. (Biology).
  3. Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:788-803.
  4. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
  5. Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation.. Cytometry. 1995; 20(2):127-33. (Biology). View Reference
  6. Schwinzer R. Cluster Report: CD45/CD45R. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:628-634.
View All (6) View Less
340416 Rev. 1

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