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      Anti-Human CD63 FITC/CD123 PE/HLA-DR PerCP

      BD FastImmune™ Anti-Human CD63 FITC/CD123 PE/HLA-DR PerCP

      (RUO (GMP))
      Product Details
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      BD FastImmune™
      Human
      Flow cytometry
      RUO (GMP)
      Phosphate buffered saline with BSA and 0.1% sodium azide.


      Description

      The CD63 antibody, clone H5C6, is derived from the fusion of P3x653 Ag8 myeloma cells with splenocytes from BALB/c mice immunized with human splenic adherent cells.

      The CD123 antibody, clone 9F5, is derived from fusion of NS-1 cells with splenocytes from BALB/c mice immunized with IL-3Rα transfected COS cells.

      Anti–HLA-DR, clone L243, is derived from the hybridization of NS-1/1-Ag4 mouse myeloma cells with spleen cells from BALB/c mice immunized with the human lymphoblastic B-cell line RPMI 8866.

      The CD63 antibody reacts with a 53-kilodalton (kDa), type III lysosomal glycoprotein found in many cell types, including monocytes, platelets, and basophils. This molecule is also referred to as LIMP, gp55, melanoma-associated antigen ME491, Pltgp40, LAMP- 3, and is a member of the tetraspannin transmembrane 4 superfamily (TM4SF).

      he CD123 antibody (Anti–IL-3Rα) binds to the α-chain of the interleukin-3 receptor (IL-3Rα). It selectively recognizes COS cells that have been transfected with IL-3Rα. Clone 9F5 does not block IL-3 binding to the receptor.

      Anti–HLA-DR recognizes a human class II major histocompatibility complex (MHC) antigen. The antigen is a transmembrane glycoprotein composed of α and β subunits that have molecular weights of 36 and 27 kilodaltons (kDa), respectively.

      Preparation And Storage

      Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

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      341068 Rev. 1
      Components
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      Description Clone Isotype EntrezGene ID
      Anti-HLA-DR PerCP L243 IgG2a, κ N/A
      CD123 PE 9F5 IgG1, κ 3563
      CD63 FITC N/A N/A N/A
      341068 Rev. 1
      Citations & References
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      Development References (31)

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      17. O'Doherty U, Peng M, Gezelter S, et al. Human blood contains two subsets of dendritic cells, one immunologically mature and the other immature.. Immunology. 1994; 82(3):487-93. (Biology). View Reference
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      341068 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

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