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BD OneFlow™ Plasma Cell Screening Tube (PCST)
Intended as an aid in the diagnosis of hematological disorders
Overview
The BD OneFlow™ PCST is part of the innovative BD OneFlow™ Solution, a comprehensive set of reagents, setup beads, protocols and assay templates to reproducibly set up the flow cytometer and stain, acquire and analyze patient specimens for immunophenotyping of normal and aberrant cell populations. It is built on the research and validation work of the EuroFlow™ Consortium on the characterization of hematological malignancies for improved accurate diagnosis.1*
The BD OneFlow™ PCST is intended for flow cytometric immunophenotyping of normal polyclonal and aberrant plasma cell populations in bone marrow as an aid in the diagnosis of hematological disorders.
Other reagents belonging to the BD OneFlow™ Solution are BD OneFlow™ Lymphoid Screening Tube (LST) , BD OneFlow™ B-cell Chronic Lymphoproliferative Diseases Tube 1 (B-CLPD T1), BD OneFlow™ Plasma Cell Disorders (PCD) Tube and BD OneFlow™ Acute Leukemia Orientation Tube (ALOT).
View videos below describing how standardization, efficiency, accuracy and reproducibility can be achieved using the BD OneFlow™ Solution on the BD FACSCanto™ II Flow Cytometer.
FEATURES
The BD OneFlow™ PCST:
- Is a preconfigured single-use 8-color reagent comprising two tubes—one containing the cytoplasmic markers (C tube) and the other containing the surface markers (S tube)
Is available in 10 test/box size (4 pouches of 5 tubes each: 2 pouches of S tubes and 2 pouches of C tubes)
Allows for easy visual identification with dark green color-coded boxes, pouches and tubes
The BD OneFlow™ Reagents increase efficiency by simplifying instrument standardization, reducing the time to prepare the instrument as well as the technical burden and training needs.2
For BD FACSLyric™ Flow Cytometer users:
- BD® CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility through Universal Setup
- BD® FC Beads (7-Color, 5-Color and 2-Color Kits) support consistency of results, eliminating the need for using cells for compensation
- BD® CS&T Beads and BD® FC Beads kits necessary for instrument setup and compensation can be used for other BD CE-IVD assays on the BD FACSLyric™ Flow Cytometer
For BD FACSCanto™ II Flow Cytometer users:
- BD FACSDiva™ CS&T IVD Beads standardize setup and monitoring for consistent performance and ensure reproducibility with Application Settings
- BD OneFlow™ Setup Beads ensure data accuracy and reproducibility by providing assay-specific target values, as per EuroFlow™ SOPs.3
- The specifically designed BD® FC Beads 8-Color Kit eliminates the need for using single-vial reagents and cells for compensation
The BD OneFlow™ PCST reagent composition
Antibody | Fluorochrome | Clone | Tube | Target Populations |
---|---|---|---|---|
CD38 | FITC | HB7 | S | Backbone markers. Identification of plasma cells |
CD56 | PE | MY31 | S | Identification of aberrant plasma cells |
β2-Microglobulin | PerCP-Cy 5.5 | TU99 | S | Identification of aberrant plasma cells |
CD19 | PE-CY 7 | SJ25-C1 | S | Backbone markers. Identification of plasma cells. Identify and characterize mature B cells |
Anti-Kappa | APC | TB28-2 | C | Plasma cells clonality |
Anti-Lambda | APC-H7 | 1-155-2 | C | Plasma cells clonality |
CD45 | BD Horizon™ V450 | 2D1 | S | Backbone markers. Identification of plasma cells |
CD138 | BD Horizon™ V500-C | MI15 | S | Backbone marker. Identification of plasma cells |
A full description of the utility of the antibodies chosen for the BD OneFlow™ PCST is provided in the publication by the EuroFlow Consortium.1
APPLICATIONS
Laboratory report of a normal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ PCST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal polyclonal and aberrant plasma cell populations.
Laboratory report of an abnormal bone marrow specimen stained with the 8-color 8-antibody BD OneFlow™ PCST single-test reagent acquired on the BD FACSLyric™ Flow Cytometer with BD FACSuite™ Clinical Application v1.4 for the identification of normal polyclonal and aberrant plasma cell populations.
Periodic and daily setup
- Instructions for Use
- Application Guides - For BD FACSLyric™ Flow Cytometer Users
- Applications Guides - For BD FACSCanto™ II Flow Cytometer Users
- Brochure
- Tools
- Instructions for Use
- Application Guides - For BD FACSLyric™ Flow Cytometer Users
- Application Guides - For BD FACSCanto™ II Flow Cytometer Users
- Application Note
BD FACSCanto™ II Flow Cytometer Videos
Standardization:
BD OneFlow™ Solution on the BD FACSCanto™ II Flow Cytometer >
Efficiency:
BD OneFlow™ Solution on the BD FACSCanto™ II Flow Cytometer >
Accuracy:
BD OneFlow™ Solution on the BD FACSCanto™ II Flow Cytometer >
Reproducibility:
BD OneFlow™ Solution on the BD FACSCanto™ II Flow Cytometer >
References
- van Dongen JJM, Lhermitte L, Böttcher S, et al. on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26(9): 1908-1975. doi: 10.1038/leu.2012.120
- Moloney E, Watson H, Barge D, et al. Efficiency and health economic evaluations of BD OneFlow™ Flow Cytometry Reagents for diagnosing chronic lymphoid leukemia. Cytometry B Clin Cytom. 2019;96(6):514-520. doi: 10.1002/cyto.b.21779
- Kalina T, Flores-Montero J, van der Velden VHJ, et al. EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols. Leukemia. 2012;26(9):1986-2010. doi: 10.1038/leu.2012.122
BD Flow Cytometers are Class 1 Laser Products. The EuroFlow trademark is the property of the EuroFlow Consortium and cannot be reproduced or published without prior written permission from the EuroFlow coordinator (www.euroflow.org). Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva. The BD OneFlow™ Solution is intended for professional use only. *This publication refers to EuroFlow and is relevant to the BD OneFlow™ Solution as the BD OneFlow™ Solution demonstrated equivalent results to EuroFlow through a clinical trial (BD OneFlow™ PCST Instructions for Use). |