Purified Mouse anti-Tyk2 (pY1054/pY1055)

BD Pharmingen™ Purified Mouse anti-Tyk2 (pY1054/pY1055)

Name: Purified Mouse anti-Tyk2 (pY1054/pY1055)
Brand: BD Pharmingen™
SKU: 558394

Clone I114-617

(RUO)

Western blot analysis of Tyk2 (pY1054/pY1055) in human Burkitt's lymphoma. Lysates from control (left panel) and IFN-α-activated (right panel) Daudi cells were probed with purified mouse anti-Tyk2 (pY1054/pY1055) monoclonal antibody) at concentrations of 4, 2, and 1 µg/ml (Lanes 1, 2, and 3, respectively). Tyk2 (pY1054/pY1055) is identified as a band of 135 kDa in the treated cells.

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Product Details

Brand: BD Pharmingen™

Reactivity: Human (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Phosphorylated peptide of the region including the tyrosines 1054 and 1055 of human Tyk2

Application: Western blot (Routinely Tested)

Target Molecular Weight: 135 kDa

Concentration: 0.5 mg/ml

RRID: AB_647302

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

558394 Rev. 2

Antibody Details

I114-617

Tyk2 is a widely expressed protein tyrosine kinase (PTK) of 1187 amino acids (135 kDa). The N-terminal kinase-related domain of Tyk2 shows 54% identity to the kinase-related domain of the JAK1 kinase. Surprisingly, the catalytic region of JAK1 shares more sequence identity to Tyk2 than it does with JAK2 kinase. The similarity of structure between Tyk2 and JAK1 suggests that both may be involved in related phosphorylation cascades. Tyk2 plays a critical role in the interferon (IFN)-α/β response while JAK1 shows involvement in both the IFN- α/β and IFN-γ signal transduction pathways. JAK2, however, appears to be required only in the IFN-γ response. It has been suggested that a portion of the large extracatalytic domain of Tyk2 could interact with components of the IFN α/β receptor.

The I114-617 monoclonal antibody recognizes the phosphorylated Y1054 and Y1055 in the tyrosine kinase domain of Tyk2.

558394 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

558394 Rev.2

Citations & References

Development References (1)

Gauzzi MC, Velazquez L, McKendry R, Mogensen KE, Fellous M, Pellegrini S. Interferon-alpha-dependent activation of Tyk2 requires phosphorylation of positive regulatory tyrosines by another kinase. J Biol Chem. 1996; 271(34):20494-20500. (Biology).

558394 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.