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Purified Mouse Anti-IKKγ
Purified Mouse Anti-IKKγ
Western blot analysis of IKKγ. Lysates from NIH/3T3 mouse embryonic fibroblast cells were probed with anti-IKKγ antibody (clone C73-764) at a concentration of 0.5 µg/ml. IKKγ is identified as a protein of 50-55 kDa (doublet).
Western blot analysis of IKKγ. Lysates from NIH/3T3 mouse embryonic fibroblast cells were probed with anti-IKKγ antibody (clone C73-764) at a concentration of 0.5 µg/ml. IKKγ is identified as a protein of 50-55 kDa (doublet).
Product Details
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BD Pharmingen™
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG1, κ
Recombinant Human IKKγ
Western blot (Routinely Tested), Immunoprecipitation (Reported)
50-55 kDa doublet
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis and immunoprecipitation. In immunoprecipitation experiments, it is reported that the C73-764 antibody brings down the IKK complex (IKKα, IKKβ, and IKKγ). The immunoprecipitation application has not been evaluated at BD Pharmingen. NIH/3T3 cells (ATCC CRL 1658) are recommended as a western blot positive control.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559675 Rev. 4
Antibody Details
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The transcription factor NF-κB, (nuclear factor kappa-B)  is controlled by interaction with an inhibitory subunit, IκB, which restricts NF-κB to the cytoplasm. Following stimulation by various cytokines or other stimuli, IκB becomes degraded and NF-κB is released to the nucleus. The release of IκB from NF-κB is a critical step in the activation of NF-κB signal pathways. A group of proteins form an NF-κB regulatory complex, or signalsome.  Two members of this complex are a pair of closely related serine/threonine kinases, IKKα and IKKβ (aka IKK-1 and IKK-2), which phosphorylate critical residues of IκB, thus targeting it for subsequent degradation. The IKK complex contains similar amounts of IKKα, IKKβ, as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKγ.  IKKα and IKKβ become activated following phosphorylation by upstream kinases, including NF-κB-inducing kinase (NIK) and MEKK1.  IKKγ interacts preferentially with IKKβ and is required for the activation of the IKK complex.  Thus the IKK kinases play an important role in the activation of NF-κB. IKKγ migrates as a doublet between 50-55 kDa in SDS-PAGE.

559675 Rev. 4
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
559675 Rev.4
Citations & References
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View product citations for antibody "559675" on CiteAb

Development References (6)

  1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature. 1997; 388(6642):548-554. (Biology). View Reference
  2. Li ZW, Chu W, Hu Y, et al. The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis. J Exp Med. 1999; 189(11):1839-1845. (Clone-specific: Immunoprecipitation). View Reference
  3. Ling L, Cao Z, Goeddel DV. NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176. Proc Natl Acad Sci U S A. 1998; 95(7):3792-3797. (Biology). View Reference
  4. Mercurio F, Zhu H, Murray BW, et al. IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation. Science. 1997; 278(5339):860-866. (Biology). View Reference
  5. Nakano H, Shindo M, Sakon S, et al. Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl Acad Sci U S A. 1998; 95(7):3537-3542. (Biology). View Reference
  6. Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature. 1998; 395(6699):297-300. (Biology). View Reference
View All (6) View Less
559675 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.