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      Purified Mouse anti-PDGFRβ (CD140b) (pY857)
      Purified Mouse anti-PDGFRβ (CD140b) (pY857)
      Western blot analysis of PDGFRβ (pY857).  Lysates from control (lanes 1-3) and PDGF-treated (lanes 4-6) NIH/3T3 mouse embryonic fibroblasts were probed with purified mouse anti-PDGFRβ (CD140b) (pY857) at concentrations of 0.25, 0.125, and 0.0625 µg/ml (lanes 1 and 4, 2 and 5 , and 3 and 6, respectively).  PDGFRβ (pY857) is identified as a band of 180 kDa in the treated cells.
      Purified Mouse anti-PDGFRβ (CD140b) (pY857)
      PDGFRβ (pY857) staining on tonsil.  Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed.  Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel).  The tissue sections were stained with purified Mouse anti-PDGFRβ (CD140b) (pY857) with Hematoxylin counterstaining.  Original magnification: 20X.
      Purified Mouse anti-PDGFRβ (CD140b) (pY857) Purified Mouse anti-PDGFRβ (CD140b) (pY857)
      Western blot analysis of PDGFRβ (pY857).  Lysates from control (lanes 1-3) and PDGF-treated (lanes 4-6) NIH/3T3 mouse embryonic fibroblasts were probed with purified mouse anti-PDGFRβ (CD140b) (pY857) at concentrations of 0.25, 0.125, and 0.0625 µg/ml (lanes 1 and 4, 2 and 5 , and 3 and 6, respectively).  PDGFRβ (pY857) is identified as a band of 180 kDa in the treated cells.
      PDGFRβ (pY857) staining on tonsil.  Fresh human tonsil was incubated in 5 mM Pervanadate solution for 2 hours, then fixed in formalin and processed.  Following antigen retrieval with BD Retrievagen A buffer (Cat. no. 550524), the sections were either left untreated (left panel) or treated with a phosphatase to eliminate all phosphorylation (right panel).  The tissue sections were stained with purified Mouse anti-PDGFRβ (CD140b) (pY857) with Hematoxylin counterstaining.  Original magnification: 20X.
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing), Human (Tested in Development)
      Mouse BALB/c IgG1, κ
      Phosphorylated Human PDGFRβ
      Western blot (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development)
      180 kDa
      0.5 mg/ml
      AB_647268
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      558360 Rev. 3
      Antibody Details
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      J24-425

      Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase.  PDGFR is composed of PDGFRα (CD140a) and/or PDGFRβ (CD140b) polypeptides.  Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to αα PDGFR, PDGF-AB binds to both αα and αβ PDGFR, and PDGF-BB binds to all three PDGFRs.  Ligand binding induces dimerization and activation of the receptor.  Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated.  PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-γ.

      The J24-425 monoclonal antibody recognizes the phosphorylated tyrosine 857 (pY857) in the tyrosine kinase domain 2 of CD140b, which is required for maximal receptor kinase activity.  The orthologous phosphorylation site in mouse PDGFRβ is Y856.

      558360 Rev. 3
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      558360 Rev.3
      Citations & References
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      Development References (4)

      1. Baxter RM, Secrist JP, Vaillancourt RR, Kazlauskas A. Full activation of the platelet-derived growth factor β-receptor kinase involves multiple events. J Biol Chem. 1998; 273(27):17050-17055. (Biology).
      2. Chiarugi P, Cirri P, Taddei ML, Giannoni E, et al. Insight into the role of low molecular weight phosphotyrosin phosphatase (LMW-PTP) on platelet-derived growth factor receptor (PDGF-r) signaling. J Biol Chem. 2002; 277(40):37331-37338. (Biology).
      3. Claesson-Welsh L. Platelet-derived growth factor receptor signals. J Biol Chem. 1994; 269(51):32023-32026. (Biology).
      4. Liu J, Oh P, Horner T, Rogers RA, Schnitzer JE. Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains. J Biol Chem. 1997; 272(11):7211-7222. (Biology). View Reference
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      558360 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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