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Purified Mouse Anti-A-Raf
Purified Mouse Anti-A-Raf
Western blot analysis of A-Raf on SKN, a human neuroblastoma, cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- A-Raf antibody.
Purified Mouse Anti-A-Raf
Immunoflourescence staining of rabbit kidney.
Western blot analysis of A-Raf on SKN, a human neuroblastoma, cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- A-Raf antibody.
Immunoflourescence staining of rabbit kidney.
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse,Rat,Dog,Chicken (Tested in Development)
Mouse IgG1
Mouse A-Raf aa. 183-199
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
68 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610074 Rev. 1
Antibody Details
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A-Raf, a proto-oncogene that encodes a 68 kDa serine/threonine-specific protein kinase, shows strong homology to c-Raf and v-Raf in the kinase region of the molecule. c-Raf appears to be uniformly and ubiquitously expressed, but A-Raf expression is much more variable. A-Raf is found at highest levels in urogenital and kidney tissues, with the lowest levels found in brain. Cell transformation by A-Raf has been shown to be independent of p21[ras], suggesting the possibility of an independent signal transduction pathway.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610074 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610074 Rev.1
Citations & References
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View product citations for antibody "610074" on CiteAb

Development References (4)

  1. Gomez E, Pritchard C, Herbert TP. cAMP-dependent protein kinase and Ca2+ influx through L-type voltage-gated calcium channels mediate Raf-independent activation of extracellular regulated kinase in response to glucagon-like peptide-1 in pancreatic beta-cells. J Biol Chem. 2002; 277(50):48146-48151. (Biology: Immunoprecipitation). View Reference
  2. Huleihel M, Goldsborough M, Cleveland J, Gunnell M, Bonner T, Rapp UR. Characterization of murine A-raf, a new oncogene related to the v-raf oncogene. Mol Cell Biol. 1986; 6(7):2655-2662. (Biology). View Reference
  3. Rapp UR. Cold Spring Harbor Symposium on Quantitative Biology. 1988:173.
  4. Storm SM, Cleveland JL, Rapp UR. Expression of raf family proto-oncogenes in normal mouse tissues. Oncogene. 1990; 5(3):345-351. (Biology). View Reference
View All (4) View Less
610074 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.