Preparation And Storage
Recommended Assay Procedures
ELISA: Purified Hamster Anti-Mouse IL-1β (Cat. No. 550605) has been reported to be useful as a capture antibody for sandwich ELISA measuring mouse IL-1β protein levels. For measuring mouse IL-1β in complex biological samples, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse IL-1β ELISA Set (Cat. No 559603).
Immunoprecipitation: Purified Hamster Anti-Mouse IL-1β (clone B122) has been reported to be useful for the immunoprecipitation of both natural and recombinant forms of mouse IL-1β protein. Please note that this antibody is not routinely tested for this application.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The B122 antibody recognizes the precursor and mature secreted forms of the mouse IL-1β protein. B122 neutralizes the biological activity of mouse IL-1β, but not the biological activities of mouse IL-1α nor human IL-1β. B122 does not recognize mouse IL-2, IL-4, IL-6, IL-10, TNF or IFN-γ. This antibody also recognizes rat IL-1β. The immunogen used to generate the B122 hybridoma was a purified, mature form of the mouse IL-1β protein.
Development References (6)
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Fei R, Gott K, Edwards B, Schuyler M. Experimental hypersensitivity pneumonitis: in vitro effects of interleukin-2 and interferon-gamma. J Lab Clin Med. 1995; 126(5):485-494. (Clone-specific: ELISA). View Reference
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Hogquist KA, Nett MA, Sheehan KC, Pendleton KD, Schreiber RD, Chaplin DD. Generation of monoclonal antibodies to murine IL-1 beta and demonstration of IL-1 in vivo. J Immunol. 1991; 146(5):1534-1540. (Clone-specific: ELISA, Immunoprecipitation). View Reference
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Kitamura T, Takaku F, Miyajima A. IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1. Int Immunol. 1991; 3(6):571-577. (Biology). View Reference
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Miller BE, Krasney PA, Gauvin DM, et al. Inhibition of mature IL-1 beta production in murine macrophages and a murine model of inflammation by WIN 67694, an inhibitor of IL-1 beta converting enzyme. J Immunol. 1995; 154(3):1331-1381. (Clone-specific: ELISA). View Reference
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Troy CM, Stefanis L, Prochiantz A, Greene LA, Shelanski ML. The contrasting roles of ICE family proteases and interleukin-1beta in apoptosis induced by trophic factor withdrawal and by copper/zinc superoxide dismutase down-regulation. Proc Natl Acad Sci U S A. 1996; 93(11):5635-5640. (Biology). View Reference
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Wu X, Wittwer AJ, Carr LS, Crippes BA, DeLarco JE, Lefkowith JB. Cytokine-induced neutrophil chemoattractant mediates neutrophil influx in immune complex glomerulonephritis in rat. J Clin Invest. 1994; 94(1):337-344. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.