Preparation And Storage
Recommended Assay Procedures
ELISA Capture: The purified C4 antibody (Cat. No. 555072) can be used as the capture antibody in a sandwich ELISA for measuring rat MCP-1 protein levels in conjunction with the biotinylated B4 antibody (Cat. No. 555074) as the detecting antibody and recombinant rat MCP-1 protein (Cat. No. 555110) as the standard. This purified capture antibody should be titrated in the range of 2 to 10 µg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of rat MCP-1 protein ranging from ~2,000 to 5 pg/ml should be used. For specific methodology, please visit our web site, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook. This ELISA pair shows no cross-reactivity with rat MIP-1α, MIP-1β, RANTES, MIP-2 or KC.
This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring MCP-1 in serum or plasma the rat MCP-1 BD OptEIA™ ELISA Set, Cat. No. 555130, is specially formulated and recommended.
IP/WB: The C4 antibody has been reported to be useful for immunoprecipitation and Western blot. Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The C4 antibody reacts with rat monocyte chemoattractant protein 1 (MCP-1). The C4 antibody shows no crossreactivity with rat MIP-1α, MIP-1β, RANTES, MIP-2 or KC. The immunogen used to generate this hybridoma was recombinant rat MCP-1 protein.
Development References (2)
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Sakanashi Y, Takeya M, Yoshimura T, Feng L, Morioka T, Takahashi K. Kinetics of macrophage subpopulations and expression of monocyte chemoattractant protein-1 (MCP-1) in bleomycin-induced lung injury of rats studied by a novel monoclonal antibody against rat MCP-1. J Leukoc Biol. 1994; 56(6):741-750. (Clone-specific: ELISA, Immunoprecipitation, Western blot). View Reference
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Yoshimura T, Takeya M, Takahashi K. Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines. Biochem Biophys Res Commun. 1991; 174(2):504-509. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.