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Sphero™ Rainbow Fluorescent Particles, 3.0 - 3.4 µm
Measurement of Rainbow Fluorescent Particle emission analyzed on a BD FACSVantage™ SE flow cytometry system. The panels depict the emission signal intensity of the Rainbow Particles following excitation by 488-nm (bottom row) and 633-nm (top row with forward scatter) wavelength light. The corresponding fluorochromes are indicated in the figure. The filter used for each emission channel is in the corresponding axis legend.
Measurement of Rainbow Fluorescent Particle emission analyzed on a BD FACSVantage™ SE flow cytometry system. The panels depict the emission signal intensity of the Rainbow Particles following excitation by 488-nm (bottom row) and 633-nm (top row with forward scatter) wavelength light. The corresponding fluorochromes are indicated in the figure. The filter used for each emission channel is in the corresponding axis legend.
Product Details
Product Details
Description
The vial contains a single population of Rainbow Particles that are dyed to a single fluorescent intensity. The intensity of this particle corresponds to the brightest intesity of peak in the Rainbow Calibration Particles, 3.0-3.4 µm (Cat. No. 556286). Every Rainbow Particle contains a mixture of fluorophores that are excited at any wavelength from 365-650 nm. The Rainbow Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis.
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Preparation and StorageStore undiluted at 2-8 °C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures
This particle mixture (~10x10^6 particles/ml) is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
556291 Rev. 8
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.