The 11G2 monoclonal antibody specifically binds to CD156c which is also known as Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10). CD156c is a ~70 kDa type I transmembrane glycoprotein that belongs to the ADAM family. It is widely expressed on hematopoietic and non-hematopoietic cells. CD156c is expressed on the plasma membrane, as well as by membranes within intracellular compartments and exosomes. CD156c serves as a broadly-reactive endopeptidase that cleaves membrane-bound proteins in a process known as ectodomain shedding or release. It can cleave transmembrane molecules into soluble forms and thereby regulate the functions of a variety of different receptors and ligands that are involved in cellular signaling and adhesion. These include membrane TNF, CX3CL1, CXCL16, Ephrin-A2, Notch and Delta molecules, cadherins, CD23, CD44, IL-6 Receptor/CD126, CD171, and amyloid precursor protein. CD156c expression may be constitutive or induced, such as in inflamed tissues, including arthritic joints and in the nervous system. Dysregulated CD156c expression may be associated with certain cancers and Alzheimer's disease.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.