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Purified Rat IgG2a, κ Isotype Control
Product Details
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BD Pharmingen™
anti-KLH
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Trinitrophenol-KLH
ELISA Standard (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
AB_395189
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

This immunoglobulin is useful as a standard in ELISA or as an isotype-matched negative control for immunofluorescent staining. An isotype control should be used at the same concentration as the antibody of interest (e.g., .1 µg /million cells for flow cytometry). We recommend the rat IgG2a, κ mAb R35-95 (Cat. No. 559073) for immunohistochemical or immunocytochemical staining.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. An isotype control should be used at the same concentration as the antibody of interest.
553992 Rev. 2
Antibody Details
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A110-2

The A110-2 antibody is specific for keyhole limpet hemocyanin (KLH)

553992 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553992 Rev.2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.