Preparation And Storage
Recommended Assay Procedures
Other reported applications include immunoprecipitation, complement-mediated depletion, and immunohistochemical staining of frozen sections.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Companion Products
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The 74-22-15A (switch variant of 74-22-15) monoclonal antibody, an isotype class-switch variant of mAb 74-22-15, specifically binds to a 230-kDa protein expressed by most pig macrophages, peripheral blood monocytes and granulocytes, and few lymphocytes. mAb 74-22-15A does not crossreact with human or bovine cells. This clone was clustered as anti-SWC3a at the First International Swine CD workshop.
Development References (8)
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Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Clone-specific: Immunohistochemistry). View Reference
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Blecha F, Kielian T, McVey DS. Workshop studies on monoclonal antibodies reactive against porcine myeloid cells. Vet Immunol Immunopathol. 1994; 43(1-3):269-272. (Biology). View Reference
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Haverson K, Bailey M, Higgins VR, Bland PW, Stokes CR. Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues. J Immunol Methods. 1994; 170(2):233-245. (Biology). View Reference
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McCullough KC, Schaffner R, Natale V, Kim YB, Summerfield A. Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages. Vet Immunol Immunopathol. 1997; 58(3-4):265-275. (Biology). View Reference
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Pescovitz MD, Lunney JK, Sachs DH. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984; 133(1):368-375. (Immunogen: Immunoprecipitation). View Reference
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Summerfield A, Haverson K, Thacker E, McCullough KC. Differentiation of porcine myeloid bone marrow haematopoietic cell populations. Vet Immunol Immunopathol. 2001; 80(1-2):121-129. (Biology). View Reference
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Sundt TM, Arn JS, Sachs DH. Patterns of T cell-accessory cell interaction in the generation of primary alloresponses in the pig. Transplantation. 1992; 54(5):911-916. (Clone-specific: Depletion). View Reference
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Yang H, Parkhouse RM. Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues. Immunology. 1996; 89(1):76-83. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.