Purified Mouse Anti-Human Vβ8

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BD Pharmingen™ Purified Mouse Anti-Human Vβ8

Name: Purified Mouse Anti-Human Vβ8
Brand: BD Pharmingen™
SKU: 555604

Clone JR2

(RUO)

Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA)

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Scientific Resources

Product Details

Brand: BD Pharmingen™

Alternative Name: BV8; TCR Vβ8; TCR V beta 8; TCR Vb8

Reactivity: Human (QC Testing)

Isotype: Mouse IgG2b, κ

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

Workshop Number: 7

RRID: AB_395975

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

FITC Goat Anti-Mouse IgG/IgM RUO

Size 0.5 mg Cat No. 555988

Purified Mouse IgG2b κ Isotype Control RUO

Size 0.1 mg Cat No. 555740

555604 Rev. 10

Antibody Details

JR2

The JR2 monoclonal antibody specifically recognizes the β-chain variable region (Vβ8) of the T-cell antigen receptor expressed on 0.5% to 3% of peripheral blood T cells. This antibody has been further characterized as having specificity to a major subset of the BV8 region as determined in the TCR Workshop of July 1995. Staphylococcal enterotoxin E has been shown to specifically stimulate T cells bearing Vβ8 (BV8).

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555604 Rev. 10

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

555604 Rev.10

Citations & References

Development References (3)

Akolkar PN, Gulwani-Akolkar B, Pergolizzi R, Bigler RD, Silver J. Influence of HLA genes on T cell receptor V segment frequencies and expression levels in peripheral blood lymphocytes. J Immunol. 1993; 150(7):2761-2773. (Biology). View Reference
Jack AS, Boylston AW, Carrel S, Grigor I. Cutaneous T-cell lymphoma cells employ a restricted range of T-cell antigen receptor variable region genes. Am J Pathol. 1990; 136(1):17-21. (Biology). View Reference
Kappler J, Kotzin B, Herron L, et al. V beta-specific stimulation of human T cells by staphylococcal toxins.. Science. 1989; 244(4906):811-3. (Biology). View Reference

555604 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.