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      Purified Mouse Anti-Human CD177
      Product Details
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      BD Pharmingen™
      Human neutrophil alloantigen 2a; HNA-2a; NB1; PRV-1; PRV1
      Human (QC Testing)
      Mouse IgG1, κ
      Human Granulocytes
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      VI M-M17, BP-609; VII 70510
      AB_394289
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      551899 Rev. 3
      Antibody Details
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      MEM-166

      The MEM-166 monoclonal antibody specifically binds to CD177 which is also known as NB1 (Neutrophil-specific antigen), HNA-2a (Human neutrophil alloantigen 2a), and PRV1 (Polycythemia rubra vera 1). CD177 is a glycophosphatidylinositol (GPI)-anchored plasma membrane glycoprotein (56-64 kDa). CD177 is expressed on subpopulations of neutrophils, neutrophillic metamyelocytes and myelocytes from 89 to 97% of healthy individuals. It is also expressed on secondary granules. Its expression is upregulated on granulocytes stimulated with the chemotactic peptide f-met-leu-phe (fMLP). Although the function of CD177 has not been fully established, some reports suggest its possible role as receptor molecule involved in leucocyte migration.

      551899 Rev. 3
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      551899 Rev.3
      Citations & References
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      View product citations for antibody "551899" on CiteAb

      Development References (4)

      1. Clement LT, Lehmeyer JE, Gartland GL.. Identification of neutrophil subpopulations with monoclonal antibodies. Blood. 1983 February; 61(2):326-332. (Biology). View Reference
      2. Matsuo K, Lin A, Procter JL, Clement L, Stroncek D. Variations in the expression of granulocyte antigen NB1. Transfusion. 2000 June; 40(6):654-662. (Biology). View Reference
      3. Stroncek DF, Shankar R, Litz C, Clement L. The expression of the NB1 antigen on myeloid precursors and neutrophils from children and umbilical cords. Transfus Med. 1998 June; 8(2):119-123. (Biology). View Reference
      4. Stroncek DF, Shankar RA, Noren PA, Herr GP, Clement LT. Analysis of the expression of NB1 antigen using two monoclonal antibodies. Transfusion. 1996 February; 36(2):168-174. (Biology). View Reference
      View All (4) View Less
      551899 Rev. 3

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.