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      Purified Mouse Anti-Human CD15s
      Product Details
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      BD Pharmingen™
      Sialyl Lewis X
      Human (QC Testing)
      Mouse BALB/c IgM, κ
      Membrane proteins from human tomach adenocarcinoma tissue
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      AB_394156
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      Functional Studies: Anti-Sialyl-Lex monoclonal antibody (clone CSLEX1) inhibits HL-60 or LEC-11 cell adhesion to

      human umbilical vein endothelial cells (HUVECs), HL-60 cell adhesion to ELAM-1-coated plates. It inhibits PMN

      and HL-60 cell adhesion to thrombin-activated platelets, or HL-60 and NewLewis CHO cell binding to GMP-140-

      coated plates. We recommend to do dialysis to remove the azide for funtional studies.

      Immunostaining: Anti-Sialyl-Le[x] monoclonal antibody (clone CSLEX1) can be used in immunoperoxidase staining of

      formalin-fixed frozen tissue sections, and immunogold staining of PMN for transmission electron microscopy. This

      monoclonal antibody can also be used to stain cells for indirect immunofluorescence microscopy.

      Immunoblotting: Anti-Sialyl-Le[x] monoclonal antibody (clone CSLEX1) can be used to detect SLex in PMN lysates by

      Western blotting.

      Enzyme-linked immunosorbent assay (ELISA)/Radioimmunoassay (RIA): Anti-Sialyl-Le[x] monoclonal antibody

      (clone CSLEX1) can be used to detect Slex on intact cells and in membrane fractions from PMN and tumor cells. This

      monoclonal antibody can also be used in solid-phase RIA.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
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      551344 Rev. 6
      Antibody Details
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      CSLEX1

      The CSLEX1 monoclonal antibody specifically binds to CD15s. This anti-Sialyl-Le[x] monoclonal antibody is specific for the α2-3 sialosylated form of lacto-N-fucopentaose III, sialyl Lex (Sle[x]). Sle[x] is expressed on granulocytes, monocytes and both normal and tumor cells of diverse origin. It has been shown to be a ligand for both endothelial leukocyte adhesion molecule-1 (ELAM-1 or E-selectin), and granule membrane protein-140 (GMP-140 or P-selectin).

      551344 Rev. 6
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      551344 Rev.6
      Citations & References
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      View product citations for antibody "551344" on CiteAb

      Development References (12)

      1. Corral L, Singer MS, Macher BA, Rosen SD. Requirement for sialic acid on neutrophils in a GMP-140 (PADGEM) mediated adhesive interaction with activated platelets. Biochem Biophys Res Commun. 1990; 172(3):1349-1356. (Biology). View Reference
      2. Dejana E, Bertocchi F, Bortolami MC. Interleukin 1 promotes tumor cell adhesion to cultured human endothelial cells. J Clin Invest. 1988; 82(4):1466-1470. (Biology). View Reference
      3. Fukushima K, Hirota M, Terasaki PI. Characterization of sialosylated Lewisx as a new tumor-associated antigen. Cancer Res. 1984; 44(11):5279-5285. (Biology). View Reference
      4. Hession C, Osborn L, Goff D. Endothelial leukocyte adhesion molecule 1: direct expression cloning and functional interactions. Proc Natl Acad Sci U S A. 1990; 87(5):1673-1677. (Biology). View Reference
      5. Phillips ML, Nudelman E, Gaeta FC, et al. ELAM-1 mediates cell adhesion by recognition of a carbohydrate ligand, sialyl-Lex. Science. 1990; 250(4984):1130-1132. (Biology). View Reference
      6. Picker LJ, Warnock RA, Burns AR, Doerschuk CM, Berg EL, Butcher EC. The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140. Cell. 1991; 66(5):921-933. (Biology). View Reference
      7. Polley MJ, Phillips ML, Wayner E, et al. CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x. Proc Natl Acad Sci U S A. 1991; 88(14):6224-6228. (Biology). View Reference
      8. Rice GE, Bevilacqua MP. An inducible endothelial cell surface glycoprotein mediates melanoma adhesion. Science. 1989; 246(4935):1303-1306. (Biology). View Reference
      9. Rice GE, Gimbrone MA, Bevilacqua MP. Tumor cell-endothelial interactions. Increased adhesion of human melanoma cells to activated vascular endothelium. Am J Pathol. 1988; 133(2):204-210. (Biology). View Reference
      10. Walz G, Aruffo A, Kolanus W, Bevilacqua M, Seed B. Recognition by ELAM-1 of the sialyl-Lex determinant on myeloid and tumor cells. Science. 1990; 250(4984):1132-1135. (Biology). View Reference
      11. Zetter BR. The cellular basis of site-specific tumor metastasis. N Engl J Med. 1990; 322(9):605-612. (Biology). View Reference
      12. Zhou Q, Moore KL, Smith DF, Varki A, McEver RP, Cummings RD. The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells. J Cell Biol. 1991; 115(2):557-564. (Biology). View Reference
      View All (12) View Less
      551344 Rev. 6

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.