PerCP-Cy™5.5 Rat Anti-Mouse CD146

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BD Pharmingen™ PerCP-Cy™5.5 Rat Anti-Mouse CD146

Name: PerCP-Cy™5.5 Rat Anti-Mouse CD146
Brand: PerCP-Cy™5.5 Rat Anti-Mouse CD146
SKU: 562231

Clone ME-9F1 (RUO)

Multicolor flow cytometric analysis of CD146 expression on C57BL/6 splenocytes. Spleen cells were stained with FITC Rat anti-Mouse CD49b antibody (Cat. No. 553857/561067) and either PerCP-Cy™5.5 Rat IgG2a, κ Isotype Control (Cat. No. 550765; Left Panel) or PerCP-Cy™5.5 Rat anti-Mouse CD146 antibody (Cat. No. 562231; Right Panel). Two-color flow cytometric dot plots showing the correlated expression patterns of CD49b versus CD146 (or Ig isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: S-endo 1; S-endo; Muc18; Mcam; Gicerin

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG2a, κ

Immunogen: CBA/J Mouse lymph node Cell Line

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_11154231

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Cy is a trademark of Amersham Biosciences Limited. This conjugated product is sold under license to the following patents: US Patent Nos. 5,486,616; 5,569,587; 5,569,766; 5,627,027.
Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. We recommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

PerCP-Cy™5.5 Rat IgG2a, κ Isotype Control RUO

Size 0.1 mg Cat No. 550765

FITC Rat Anti-Mouse CD49b RUO

Size 0.5 mg Cat No. 553857

FITC Rat Anti-Mouse CD49b RUO

Size 25 µg Cat No. 561067

562231 Rev. 1

Antibody Details

ME-9F1

The ME-9F1 monoclonal antibody specifically binds to mouse CD146. The CD146 adhesion molecule is a type 1 transmembrane glycoprotein and member of the immunoglobulin superfamily. CD146 is expressed by blood vessel endothelial cells and may play roles in forming intercellular junctions between endothelial cells and influencing the transendothelial migration of other cell types. CD146 is also expressed by some melanoma cell lines, NK cells and neutrophils. CD146 is not detectable on mouse monocytes, dendritic cells, T cells, NKT cells, B cells and smooth muscle cells. Increased expression of CD146 is reportedly associated with NK cell maturation and may be used to characterize different functional NK cell subsets. Activated CD146-positive mouse NK cells reportedly are less cytotoxic and secrete less IFN-γ than their CD146-negative counterparts.

562231 Rev. 1

Format Details

PerCP-Cy5.5

PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PerCP-Cy5.5

Excitation Source: Blue 488 nm

Excitation Max: 482 nm

Emission Max: 676 nm

562231 Rev.1

Citations & References

View product citations for antibody "562231" on CiteAb

Development References (4)

Alais S, Allioli N, Pujades C, et al. HEMCAM/CD146 downregulates cell surface expression of beta1 integrins. J Cell Sci. 2001; 114(Pt 10):1847-1859. (Biology). View Reference
Despoix N, Walzer T, Jouve N, et al. Mouse CD146/MCAM is a marker of natural killer cell maturation. Eur J Immunol. 2008; 38(10):2855-2864. (Biology). View Reference
Schrage A, Loddenkemper C, Erben U, et al. Murine CD146 is widely expressed on endothelial cells and is recognized by the monoclonal antibody ME-9F1. Histochem Cell Biol. 2008; 129(4):441-451. (Clone-specific: Flow cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation). View Reference
Yang H, Wang S, Liu Z, et al. Isolation and characterization of mouse MUC18 cDNA gene, and correlation of MUC18 expression in mouse melanoma cell lines with metastatic ability. Gene. 2001; 265(1-2):133-145. (Biology). View Reference

View All (4)

562231 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.