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      PE Rat Anti-Mouse Vα2 TCR
      PE Rat Anti-Mouse Vα2 TCR
      Two-color analysis of the expression of Vα2 TCR on peripheral lymphocytes. BALB/c lymph nodes cells were incubated simultaneously with PE-conjugated B20.1, FITC-conjugated RM4-5 (anti-CD4, Cat. No. 553046/553047), and FITC-conjugated 53-5.8 (anti-CD8b.2, Cat. No. 553040) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      PE Rat Anti-Mouse Vα2 TCR
      Two-color analysis of the expression of Vα2 TCR on peripheral lymphocytes. BALB/c lymph nodes cells were incubated simultaneously with PE-conjugated B20.1, FITC-conjugated RM4-5 (anti-CD4, Cat. No. 553046/553047), and FITC-conjugated 53-5.8 (anti-CD8b.2, Cat. No. 553040) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
      Soluble αβ TCR from mouse cytotoxic T-cell clone KB5-C20
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      111660
      AB_394760
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553289 Rev. 9
      Antibody Details
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      B20.1

      The B20.1 monoclonal antibody specifically binds to most members of the Vα2 T-cell Receptor (TCR) subfamily in mice having the a, b, and c haplotypes of the Tcrb gene complex. B20.1 antibody may crossreact with Vδ8 TCR, which shares >90% sequence homology with Vα2 TCR. Levels of B20.1+ T cells appear to be influenced by Vα haplotypes. Moreover, the frequencies of Vα2+ CD8+ and CD4+ T cells are influenced by H-2 haplotypes.

      553289 Rev. 9
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      553289 Rev.9
      Citations & References
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      View product citations for antibody "553289" on CiteAb

      Development References (3)

      1. Grégoire C, Rebaï N, Schweisguth F, et al. Engineered secreted T-cell receptor alpha beta heterodimers.. Proc Natl Acad Sci USA. 1991; 88(18):8077-81. (Clone-specific). View Reference
      2. Pircher H, Rebaï N, Groettrup M, et al. Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody.. Eur J Immunol. 1992; 22(2):399-404. (Immunogen). View Reference
      3. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
      553289 Rev. 9

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.