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PE Rat Anti-Mouse Galectin-9
PE Rat Anti-Mouse Galectin-9

Flow cytometric analysis of Galectin-9 expression by mouse thymocytes. C57BL/6 mouse thymocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722), and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram) or PE Rat Anti-Mouse Galectin-9 antibody (Cat. No. 566027; solid line histogram).  The fluorescence histogram showing Galectin-9 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Flow cytometric analysis of Galectin-9 expression by mouse thymocytes. C57BL/6 mouse thymocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722), and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; dashed line histogram) or PE Rat Anti-Mouse Galectin-9 antibody (Cat. No. 566027; solid line histogram).  The fluorescence histogram showing Galectin-9 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact thymocytes. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

Product Details
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BD Pharmingen™
Galectin9; Gal-9; Gal9; Lgals9; LEG9
Mouse (QC Testing)
Rat IgG2a, κ
Mouse Galectin-9 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739471
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.
566027 Rev. 1
Antibody Details
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RG9-35

The RG9-35 monoclonal antibody specifically binds to Galectin 9 (Gal-9) which is encoded by Lgals9 (lectin, galactose binding, soluble 9). Galectin-9 is expressed within cells and can be conveyed to the cell surface and released in soluble form. It is expressed by various cell types including lymphocytes, thymocytes, macrophages, dendritic cells, astrocytes, mast cells, eosinophils, fibroblasts, epithelial cells, and endothelial cells. Its expression can be induced by cells in response to proinflammatory cytokines or infection by certain viruses. Galectin-9 is a ~ 40 kDa S-type lectin that has two carbohydrate recognition domains (CRDs) and forms homodimers. It binds to β-galactosides and can serve as a ligand for TIM-3 (CD366). Galectin-9 can play multiple roles in innate and adaptive immune responses including the activation of macrophage cytokine secretion and bactericidal functions, promotion of dendritic cell maturation, regulatory T cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T cells. The RG9-35 antibody can reportedly block some biological activities of Galectin-9.

        

566027 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566027 Rev.1
Citations & References
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Development References (8)

  1. Chiba S, Baghdadi M, Akiba H, et al. Tumor-infiltrating DCs suppress nucleic acid-mediated innate immune responses through interactions between the receptor TIM-3 and the alarmin HMGB1.. Nat Immunol. 2012; 13(9):832-42. (Clone-specific: In vivo exacerbation). View Reference
  2. Fukushima A1, Sumi T, Fukuda K, et al. Roles of galectin-9 in the development of experimental allergic conjunctivitis in mice.. Int Arch Allergy Immunol. 2008; 146(1):36-43. (Immunogen: Functional assay, In vivo exacerbation). View Reference
  3. Jayaraman P, Sada-Ovalle I, Beladi S, et al. Tim3 binding to galectin-9 stimulates antimicrobial immunity.. J Exp Med. 2010; 207(11):2343-54. (Biology). View Reference
  4. Seki M, Oomizu S, Sakata KM, et al. Galectin-9 suppresses the generation of Th17, promotes the induction of regulatory T cells, and regulates experimental autoimmune arthritis.. Clin Immunol. 2008; 127(1):78-88. (Biology). View Reference
  5. Shimmura-Tomita M, Wang M, Taniguchi H, Akiba H, Yagita H, Hori J. Galectin-9-mediated protection from allo-specific T cells as a mechanism of immune privilege of corneal allografts.. PLoS ONE. 2013; 8(5):e63620. (Clone-specific: Blocking, Flow cytometry, Fluorescence microscopy, Immunofluorescence, Inhibition, In vivo exacerbation). View Reference
  6. Thornley TB, Fang Z, Balasubramanian S, et al. Fragile TIM-4-expressing tissue resident macrophages are migratory and immunoregulatory.. J Clin Invest. 2014; 124(8):3443-54. (Clone-specific: Flow cytometry, Functional assay). View Reference
  7. Wada J, Ota K, Kumar A, Wallner EI, Kanwar YS. Developmental regulation, expression, and apoptotic potential of galectin-9, a beta-galactoside binding lectin.. J Clin Invest. 1997; 99(10):2452-61. (Biology). View Reference
  8. Zhu C, Anderson AC, Schubart A, et al. The Tim-3 ligand galectin-9 negatively regulates T helper type 1 immunity.. Nat Immunol. 2005; 6(12):1245-52. (Biology). View Reference
View All (8) View Less
566027 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.