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BD Pharmingen™ PE Rat anti-Mouse CD107a
Clone 1D4B (RUO)
Flow cytometric analysis of PE-conjugated anti-mouse CD107a on mouse splenocytes.
Murine splenocytes were permeabilized with BD Cytofix/Cytoperm™ fixation/permeabilization buffer (Cat. No.554722) and then stained with either PE anti-CD107a (clone 1D4B, Cat. No. 558661, solid line) or a PE rat IgG2a isotype control (catalog number 553930, dashed line). Intracellular staining was analysed by flow cytometry performed on a BD FACSCalibur™ System. The histograms were derived from the gated events based on light scattering characteristics of viable splenocytes.
Flow cytometric analysis of PE-conjugated anti-mouse CD107a on mouse splenocytes.
Murine splenocytes were permeabilized with BD Cytofix/Cytoperm™ fixation/permeabilization buffer (Cat. No.554722) and then stained with either PE anti-CD107a (clone 1D4B, Cat. No. 558661, solid line) or a PE rat IgG2a isotype control (catalog number 553930, dashed line). Intracellular staining was analysed by flow cytometry performed on a BD FACSCalibur™ System. The histograms were derived from the gated events based on light scattering characteristics of viable splenocytes.
Product Details
BD Pharmingen™
LAMP-1; LGP-120; LGP-A; P2B
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
Plasma membrane fraction of mouse embryo NIH 3T3 cell line
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_1645247
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Fixation and Permeabilization Solution RUO
Size
125 ML
Cat No.
554722
PE Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
553930
558661 Rev. 1
Antibody Details
1D4B
The 1D4B antibody recognizes CD107a which is also known as, Lysosome-Associated Membrane Protein 1 (LAMP-1). CD107a is one of the two major glycoproteins in lysosome membranes that provide useful markers to distinguish lysosomes from other organelles. CD107a may play a role in the lysosomal degradation of certain molecules. Mouse CD107a is a type I transmembrane glycoprotein. It consists of a 40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoprotein of 110-140 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs. Following activation, CD107a is relocated to the surface of some lymphocytes, macrophages, epithelial cells, endothelial cells, platelets, and tumor cells. Cell-surface CD107a may participate in intercellular adhesion and adhesion to the extracellular matrix. Cell surface CD107a expression can serve as a useful marker for cytotoxic NK and CD8+ T cells, as well as, some malignant tumor cells.
558661 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
558661 Rev.1
Citations & References
View product citations for antibody "558661" on CiteAb
Development References (4)
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Arterburn LM, Earles BJ, August JT. The disulfide structure of mouse lysosome-associated membrane protein 1. J Biol Chem. 1990; 265(13):7419-7423. (Biology). View Reference
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Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986; 51:97-112. (Biology). View Reference
-
Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101(1):85-95. (Immunogen). View Reference
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Chen JW, Pan W, D'Souza MP, August JT. Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells. Arch Biochem Biophys. 1985; 239(2):574-586. (Biology). View Reference
558661 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.