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PE Mouse Anti-Human CD39
PE Mouse Anti-Human CD39
Flow cytometric analysis of CD39 expression on human peripheral blood leucocyte populations.        Upper Plots: Whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat No. 554680, Left Plot) or PE Mouse Anti-Human CD39 antibody (Cat No. 567156/567157, Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat No. 555899). A bivariate pseudocolor density plot showing the correlated expression of CD39 (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations.        Lower Plots: Human peripheral blood mononuclear cells (PBMC) were preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220) and then stained with FITC Mouse Anti-Human CD4 (Cat. No. 555346/561005/561842), PE-Cy™7 Mouse Anti-Human CD25 (Cat. No. 557741/560920/561405), Alexa Fluor® 647 Mouse Anti-Human CD127 (Cat No. 558598/560905) antibodies, and either PE Mouse IgG1, κ Isotype Control (dashed line histogram) or PE Mouse Anti-Human CD39 (solid line histogram). Bivariate pseudocolor density plots showing the coexpressed levels of CD25 versus CD127 by viable light scatter-gated CD4+ T cells [Left Plot] were further gated to reveal CD39 expression or Ig Isotype control staining [Right Plot] on CD4+CD25+CD127low T cells (ie, cells with a Regulatory T cell immunophenotype) as shown.        Flow cytometric analysis was performed using a BD FACSCelelsta™ or LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software.
Flow cytometric analysis of CD39 expression on human peripheral blood leucocyte populations.        Upper Plots: Whole blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat No. 554680, Left Plot) or PE Mouse Anti-Human CD39 antibody (Cat No. 567156/567157, Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat No. 555899). A bivariate pseudocolor density plot showing the correlated expression of CD39 (or Ig Isotype control staining) versus side light-scatter signals (SSC-A) was derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations.        Lower Plots: Human peripheral blood mononuclear cells (PBMC) were preincubated with BD Pharmingen™ Human BD Fc Block™ (Cat. No. 564219/564220) and then stained with FITC Mouse Anti-Human CD4 (Cat. No. 555346/561005/561842), PE-Cy™7 Mouse Anti-Human CD25 (Cat. No. 557741/560920/561405), Alexa Fluor® 647 Mouse Anti-Human CD127 (Cat No. 558598/560905) antibodies, and either PE Mouse IgG1, κ Isotype Control (dashed line histogram) or PE Mouse Anti-Human CD39 (solid line histogram). Bivariate pseudocolor density plots showing the coexpressed levels of CD25 versus CD127 by viable light scatter-gated CD4+ T cells [Left Plot] were further gated to reveal CD39 expression or Ig Isotype control staining [Right Plot] on CD4+CD25+CD127low T cells (ie, cells with a Regulatory T cell immunophenotype) as shown.        Flow cytometric analysis was performed using a BD FACSCelelsta™ or LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ software.
Product Details
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BD Pharmingen™
A1; ENTPD1; NTPDase-1; ecto-ATPDase 1; ecto-ATPase 1; ecto-apyrase
Human (QC Testing)
Mouse BALB/c IgG1, κ
PHA-activated Human Peripheral Blood Mononuclear Cells
Flow cytometry (Routinely Tested)
5 µl
V CD39.07; VI CD39,1
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
567156 Rev. 1
Antibody Details
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A1

The A1 monoclonal antibody specifically recognizes human CD39 which is also known as Ecto-ATP diphosphohydrolase 1 (Ecto-ATPase 1 or Ecto-ATPDase 1), Ecto-apyrase or NTPDase 1. CD39 is a ~78 kDa integral membrane glycoprotein that is encoded by ENTPD1 (Ectonucleoside triphosphate diphosphohydrolase 1). CD39 contains two transmembrane domains, one having a N- and the other a C-terminal cytoplasmic tail, and a large extracellular domain that has the enzymatic site. CD39 is also known as Lymphoid cell activation antigen because its expression is induced upon activation of T and B cells. CD39 is variably expressed on some regulatory T cells, NK cells, granulocytes, monocytes, dendritic cells, Langerhans cells, endothelial cells, platelets, and neurons. CD39 is a member of the ectonucleoside triphosphate dihydrolases (E-NTPDases) family that is involved in the regulation of extracellular nucleotide catabolism by controlling the extracellular nucleoside triphosphate pool (NTP). It functions as an ectoenzyme that can hydrolyze both nucleoside triphosphates and diphosphates such as ATP and ADP and thereby suppress inflammation and regulate platelet activation as well as purinergic neurotransmission. The ectoenzymes CD39 and CD73 can act in tandem to enable regulatory T cells (Treg) to generate immunosuppressive adenosine and thereby regulate immune responses.

        

567156 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
567156 Rev.1
Citations & References
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Development References (6)

  1. Allard B, Longhi MS, Robson SC, Stagg J. The ectonucleotidases CD39 and CD73: Novel checkpoint inhibitor targets.. Immunol Rev. 2017; 276(1):121-144. (Biology). View Reference
  2. Aversa GG, Suranyi MG, Waugh JA, Bishop AG, Hall BM. Detection of a late lymphocyte activation marker by A1, a new monoclonal antibody.. Transplant Proc. 1988; 20(1):49-52. (Immunogen: Flow cytometry). View Reference
  3. Aversa GG, Waugh JA, Bishop GA, Hall BM. Use of monoclonal antibodies to study in vivo and in vitro-activated lymphocytes.. Transplant Proc. 1989; 21(1 Pt 1):349-50. (Clone-specific: Flow cytometry). View Reference
  4. Gouttefangeas C, Mansur I, Bensussan A, Boumsell L. Biochemical analysis and epitope mapping of mAb defining CD39. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:383-385.
  5. Häusler SF, Del Barrio IM, Diessner J, et al. Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion.. Am J Transl Res. 2014; 6(2):129-39. (Clone-specific: Flow cytometry, Functional assay, Inhibition). View Reference
  6. Jones M, Mason DY. CD39 Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:157-159.
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567156 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.