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PE Mouse Anti-Human CD11c
PE Mouse Anti-Human CD11c

Two-parameter flow cytometric analysis of CD11c expression on human peripheral blood leucocyte populations. Human peripheral blood (collected with heparin as the preferred anticoagulant rather than EDTA) was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 557714; Left Panel) or PE Mouse Anti-Human CD11c antibody (Cat. No. 566730; Right Panel) at 0.5 μg. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter pseudocolor density plots showing the correlated expression of CD11c (or Ig Isotype control staining) versus side light scatter signals (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software. Data shown in this Technical Data Sheet are not lot specific.

Two-parameter flow cytometric analysis of CD11c expression on human peripheral blood leucocyte populations. Human peripheral blood (collected with heparin as the preferred anticoagulant rather than EDTA) was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 557714; Left Panel) or PE Mouse Anti-Human CD11c antibody (Cat. No. 566730; Right Panel) at 0.5 μg. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter pseudocolor density plots showing the correlated expression of CD11c (or Ig Isotype control staining) versus side light scatter signals (SSC-A) were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Flow Cytometer System and FlowJo™ software. Data shown in this Technical Data Sheet are not lot specific.

Product Details
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BD Pharmingen™
ITGAX; integrin alpha X; CD11C; p150 95 integrin alpha chain; SLEB6
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human Synovial Fluid Dendritic Cells
Flow cytometry (Routinely Tested)
0.2 mg/ml
III 256; V S143
AB_2869833
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

Note: The binding of the BU15 antibody to CD11c is divalent cation dependent. Therefore, heparin is recommended for use as the blood anticoagulant rather than the EDTA chelating agent that might adversely affect BU15 antibody binding and cellular staining.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566730 Rev. 2
Antibody Details
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BU15

The BU15 monoclonal antibody specifically binds to CD11c, which is also known as Integrin alpha X (αX Integrin) or p150,95 Integrin alpha chain. CD11c is a ~150 kDa type I transmembrane glycoprotein that is encoded by ITGAX (Integrin subunit alpha X) which belongs to the integrin alpha chain family. It is variably expressed on monocytes, macrophages, granulocytes, NK cells, dendritic cells, and subsets of B and T cells. CD11c associates with CD18 (Integrin beta 2/β2 Integrin) to form the heterodimeric CD11c:CD18 complex, which is also known as p150,95 Integrin, or the Type 4 Complement Receptor (CR4). CD11c:CD18 binds to fibrinogen, iC3b, ICAM-1 (CD54), or lipopolysaccharide (LPS). CD11c:CD18 functions as an adhesion molecule that mediates cellular binding to ligands expressed on stimulated cells including epithelium and endothelium found during inflammation.

        

566730 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566730 Rev.2
Citations & References
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Development References (4)

  1. Hogg N, Horton MA. Myeloid antigens: New and previously defined clusters. In: McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:576-602.
  2. Hogg N. Human mononuclear phagocyte molecules and the use of monoclonal antibodies in their detection. Clin Exp Immunol. 1987; 69(3):687-694. (Clone-specific). View Reference
  3. Luk J, Springer TA. CD11c cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1590-1592.
  4. Zola H, Swart B, Nicholson I, Voss E. CD11c. In: Zola H. Leukocyte and Stromal Cell Molecules : the CD Markers. Hoboken, N.J.: Wiley-Liss; 2007:60.
View All (4) View Less
566730 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.