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BD Pharmingen™ PE Hamster Anti-Mouse CD49b
Clone HMα2 (RUO)
Two-color analysis of CD49b expression on spleen leukocytes. BALB/c splenocytes were simultaneously stained with PE-conjugated mAb HMα2 mAb (right panel) and FITC-conjugated mAb M1/70 (anti-mouse CD11b, Cat. No. 557396/553310, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Two-color analysis of CD49b expression on spleen leukocytes. BALB/c splenocytes were simultaneously stained with PE-conjugated mAb HMα2 mAb (right panel) and FITC-conjugated mAb M1/70 (anti-mouse CD11b, Cat. No. 557396/553310, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Two-color analysis of CD49b expression on spleen leukocytes. BALB/c splenocytes were simultaneously stained with PE-conjugated mAb HMα2 mAb (right panel) and FITC-conjugated mAb M1/70 (anti-mouse CD11b, Cat. No. 557396/553310, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Two-color analysis of CD49b expression on spleen leukocytes. BALB/c splenocytes were simultaneously stained with PE-conjugated mAb HMα2 mAb (right panel) and FITC-conjugated mAb M1/70 (anti-mouse CD11b, Cat. No. 557396/553310, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
Product Details
BD Pharmingen™
Integrin α2 chain
Mouse (QC Testing)
Armenian Hamster IgG1, κ
Mouse colon carcinoma cell line Colon26
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_397108
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
PE Hamster IgG1 κ Isotype Control RUO
Size
0.1 mg
Cat No.
553972
FITC Rat Anti-CD11b RUO
Size
0.1 mg
Cat No.
557396
558759 Rev. 12
Antibody Details
HMα2
The HMα2 antibody reacts with integrin α2 chain (CD49b), the 150-kDa transmembrane glycoprotein that non-covalently associates with the integrin β1 subunit (CD29) to form the integrin α2β1 complex known as VLA-2. VLA-2, a receptor for collagen and laminin, is expressed on some splenic CD4+ T lymphocytes and NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, splenic NK cells, epithelial cells, and platelets; but it is not on thymocytes or Peyer's-patch or lymphnode lymphocytes. The expression of VLA-2 is upregulated on lymphocytes in response to mitogens. The HMα2 antibody has been reported to partially block the interaction of T-cell blasts, but not NK cells, with collagen. Purified HMα2 mAb blocks the staining of splenic NK cells by the anti-CD49b/Pan-NK Cells mAb DX5 (Cat. No. 553858, for the PE conjugate). Therefore, mAb HMα2 may be used like the DX5 mAb for identification of NK cells.
558759 Rev. 12
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
558759 Rev.12
Citations & References
View product citations for antibody "558759" on CiteAb
Development References (5)
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Arase H, Saito T, Phillips JH, Lanier LL. Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2). J Immunol. 2001; 167(3):1141-1144. (Biology). View Reference
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Chen H, Paul WE. A population of CD62Llow Nk1.1- CD4+ T cells that resembles NK1.1+ CD4+ T cells. Eur J Immunol. 1998; 28(10):3172-3182. (Biology). View Reference
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Miyake S, Sakurai T, Okumura K, Yagita H. Identification of collagen and laminin receptor integrins on murine T lymphocytes. Eur J Immunol. 1994; 24(9):2000-2005. (Immunogen). View Reference
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Noto K, Kato K, Okumura K, Yagita H. Identification and functional characterization of mouse CD29 with a mAb. Int Immunol. 1995; 7(5):835-842. (Biology). View Reference
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Tanaka T, Ohtsuka Y, Yagita H, Shiratori Y, Omata M, Okumura K. Involvement of alpha 1 and alpha 4 integrins in gut mucosal injury of graft-versus-host disease. Int Immunol. 1995; 7(8):1183-1189. (Biology). View Reference
558759 Rev. 12
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.