Flow cytometric analysis of Ig, λ1, λ2, & λ3 light chain expression on mouse splenocytes. B6 splenocytes were costained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and either FITC Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553434; Right Panel) or FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; Left Panel). Two-color dot plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACSCanto™ II system.
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Flow cytometric analysis of Ig, λ1, λ2, & λ3 light chain expression on mouse splenocytes. B6 splenocytes were costained with PE Rat Anti-Mouse CD45R/B220 (Cat. No. 553089/553090/561878), and either FITC Rat Anti-Mouse Ig, λ1, λ2, & λ3 Light Chain (Cat. No. 553434; Right Panel) or FITC Rat IgG2a, κ Isotype Control (Cat. No. 553929; Left Panel). Two-color dot plots were derived from gated events with the side and forward light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACSCanto™ II system.
Product Details
BD Pharmingen™
Igl; Ig λ; Immunoglobulin lambda chain complex; Ig λ1/λ2/λ3
Mouse (QC Testing)
Rat IgG2a, κ
Pooled Mouse Ig
Flow cytometry (Routinely Tested)
0.5 mg/ml
111519
AB_394854
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553434 Rev. 9
Antibody Details
R26-46
The R26-46 antibody reacts specifically with mouse Igs bearing λ1, λ2, or λ3 light chains. It does not react with κ light chain or heavy chain. Detection of surface immunoglobulin on Ig λ chain-secreting hybridoma cells has been demonstrated with R26-46 mAb.
553434 Rev. 9
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
553434 Rev.9
Citations & References
View product citations for antibody "553434" on CiteAb
Development References (2)
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Li Y, Li H, Weigert M. Autoreactive B cells in the marginal zone that express dual receptors.. J Exp Med. 2002; 195(2):181-8. (Clone-specific). View Reference
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Rolink AG, Winkler T, Melchers F, Andersson J. Precursor B cell receptor-dependent B cell proliferation and differentiation does not require the bone marrow or fetal liver environment.. J Exp Med. 2000; 191(1):23-32. (Clone-specific). View Reference
553434 Rev. 9
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.