The MR11-1 monoclonal antibody specifically recognizes the Vβ 12 T-cell Receptor (TCR) of mice having the b haplotype (e.g., C57BL, C58, DBA/1) of the Tcrb gene complex1 The Tcrb-V12 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) haplotype. Vβ 12 TCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and superantigens encoded by Mtv-8 (Mlsf, Dvb11.1), Mtv-9 (Etc-1, Mlsf, Dvb11.2) and/or Mtv-11 (Mlsf, Dvb11.3) proviruses (eg, A, AKR, BALB/c, CBA/J, C3H, DBA/2). Activation of Vβ 12 TCR-expressing T cells by these determinants is dependent upon presentation by I-E. C57BL/6 spleen T cells expressing Vβ 12 TCR are among the predominant responders to MAIDS virus superantigen.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.