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BV510 Mouse Anti-Human CD147
BV510 Mouse Anti-Human CD147
Flow cytometric analysis of CD147 expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ BV510 Mouse Anti-Human CD147 antibody (Cat. No. 563249; solid line histogram) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Flow cytometric analysis of CD147 expression on human peripheral blood lymphocytes. Whole blood was stained with BD Horizon™ BV510 Mouse Anti-Human CD147 antibody (Cat. No. 563249; solid line histogram) or with a BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No.555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
Product Details
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BD Horizon™
BSG; Basigin; BASI; Neurothelin; 5F7; EMMPRIN; M6; OK; TCSF
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
5 µl
VI NL109
AB_2738095
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV510 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV510 were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Brilliant Violet™ 510 is a trademark of Sirigen.
563249 Rev. 1
Antibody Details
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HIM6

The HIM6 monoclonal antibody specifically binds to CD147 which is encoded by BSG. CD147 is a type I transmembrane glycoprotein (30-50 kDa) of the immunoglobulin super-gene family. Neurothelin, a blood-brain barrier-specific molecule, was clustered as CD147 in the Sixth Human Leukocyte Differentiation Antigen (HLDA) workshop. It bears homology with mouse gp42 or basigin, human "M6" or "EMMPRIN", rat OX-47 or CD-9, and avian HT7 or 5A11. CD147 is also known as Tumor cell-derived collagenase stimulatory factor (TCSF). CD147 is a molecule that is broadly expressed on cells of hematopoietic and non-hematopoietic origin. Its expression on specific cell types may be regulated by cytokines. CD147 plays a role in embryonal blood-brain barrier development and a role in integrin-mediated adhesion in brain endothelia.

       The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.

563249 Rev. 1
Format Details
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BV510
The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV510
Violet 405 nm
327 nm, 405 nm
512 nm
563249 Rev.1
Citations & References
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View product citations for antibody "563249" on CiteAb

Development References (5)

  1. Majdic O, Pickl WF, Kohl P, Stockinger H, Knapp W. EC16.3 CD147 Workshop: Reactivity and epitope mapping of CD147 monoclonal antibodies. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:765-766.
  2. Riethdorf S, Reimers N, Assmann V, et al. High incidence of EMMPRIN expression in human tumors. Int J Cancer. 2006; 119(8):1800-1810. (Clone-specific: Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
  3. Rizzo A. Aragona E, Dino O, et al. EC16.1 CD147 Workshop: Expression of CD147 (neurothelin) in liver and lung cancer. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:763-764.
  4. Stockinger H, Ebel T, Hansmann C, et al.. EC16 CD147 (neurothelin/basigin) Workshop Panel Report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:760-763.
  5. Sudou A, Ozawa M, Muramatsu T. Lewis X structure increases cell substratum adhesion in L cells. J Biochem (Tokyo). 1995; 117(2):271-275. (Biology). View Reference
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563249 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.