The R12-31 monoclonal antibody specifically recognizes Receptor activator of NF-KB (RANK) which is also known as CD265, TNF-related activation-induced cytokine receptor (TRANCE Receptor/TRANCE-R), Osteoclast differentiation factor receptor (ODFR), or Ly109. CD265 (RANK) is a type I transmembrane glycoprotein that is encoded by Tnfrsf11a (Tumor necrosis factor receptor superfamily member 11a). CD265 (RANK) is expressed by cells in various tissues including osteoclasts and dendritic cells. CD265 (RANK) serves as the signaling receptor for RANKL (CD254/TRANCE) and regulates the activation and differentiation of osteoclasts. Signaling through the RANK:RANKL interaction also increases the survival of dendritic cells and their antigen presentation function to T cells.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.