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BD Horizon™ BV421 Mouse Anti-Human Neuropilin-1 (CD304)
Clone U21-1283 (RUO)
Two-color flow cytometric analysis of Neuropilin-1 (CD304) expression on human peripheral blood lymphoid cells. Human peripheral blood mononuclear cells were stained with APC Mouse Anti-Human CD123 antibody (Cat. No. 560087) and either BD Horizon™ BV421 Mouse IgG2b, κ Isotype Control (Cat. No. 562748; Left Plot) or BD Horizon BV421 Mouse Anti-Human Neuropilin-1 (CD304) antibody (Cat. No. 565953/565954; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Neuropilin-1 (CD304) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of Neuropilin-1 (CD304) expression on human peripheral blood lymphoid cells. Human peripheral blood mononuclear cells were stained with APC Mouse Anti-Human CD123 antibody (Cat. No. 560087) and either BD Horizon™ BV421 Mouse IgG2b, κ Isotype Control (Cat. No. 562748; Left Plot) or BD Horizon BV421 Mouse Anti-Human Neuropilin-1 (CD304) antibody (Cat. No. 565953/565954; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Neuropilin-1 (CD304) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of Neuropilin-1 (CD304) expression on human peripheral blood lymphoid cells. Human peripheral blood mononuclear cells were stained with APC Mouse Anti-Human CD123 antibody (Cat. No. 560087) and either BD Horizon™ BV421 Mouse IgG2b, κ Isotype Control (Cat. No. 562748; Left Plot) or BD Horizon BV421 Mouse Anti-Human Neuropilin-1 (CD304) antibody (Cat. No. 565953/565954; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Neuropilin-1 (CD304) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Two-color flow cytometric analysis of Neuropilin-1 (CD304) expression on human peripheral blood lymphoid cells. Human peripheral blood mononuclear cells were stained with APC Mouse Anti-Human CD123 antibody (Cat. No. 560087) and either BD Horizon™ BV421 Mouse IgG2b, κ Isotype Control (Cat. No. 562748; Left Plot) or BD Horizon BV421 Mouse Anti-Human Neuropilin-1 (CD304) antibody (Cat. No. 565953/565954; Right Plot). Two-color flow cytometric contour plots showing the correlated expression of CD123 versus Neuropilin-1 (CD304) [or Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable lymphoid cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
BD Horizon™
Neuropilin; NRP; NP1; Blood dendritic cell antigen 4; BDCA-4; VEGF165R
Human (QC Testing)
Mouse IgG2b, κ
Recombinant Human Neuropilin-1
Flow cytometry (Routinely Tested)
5 µl
AB_2744360
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Brilliant Stain Buffer RUO
Size
100 Tests
Cat No.
563794
BV421 Mouse IgG2b, κ Isotype Control RUO
Size
50 µg
Cat No.
562748
APC Mouse anti-Human CD123 RUO
Size
100 Tests
Cat No.
560087
BV421 Mouse Anti-Human Neuropilin-1 (CD304) RUO
Size
100 Tests
Cat No.
565953
565954 Rev. 1
Antibody Details
U21-1283
The U21-1283 monoclonal antibody specifically recognizes Neuropilin-1 (NRP1), also known as CD304, Blood dendritic cell antigen 4 (BDCA4), and Vascular endothelial cell growth factor 165 receptor (VEGF165R). CD304 is a type I transmembrane glycoprotein involved in the development of the nervous and cardiovascular systems. It mediates the interaction, growth, survival, and migration of a variety of normal and tumor cells. CD304 is expressed on neurons, thymocytes, regulatory T cells, a subset of T follicular helper cells, dendritic cells, endothelial cells, and certain tumor cells. Neuropilin-1 has a very short cytoplasmic domain and interacts with various coreceptors to form ligand-binding, signal-transducing receptor complexes. CD304 complexes with Plexin-A family members to bind chemorepellent Class 3 Semaphorins and guide neuronal axon growth. It also functions as a coreceptor with VEGFR2/CD309 to stimulate angiogenesis in response to VEGF165. CD304 mediates the interactions between some T cells and dendritic cells.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
565954 Rev. 1
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
565954 Rev.1
Citations & References
View product citations for antibody "565954" on CiteAb
Development References (6)
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Bruder D et al. Neuropilin-1: a surface marker of regulatory T cells.. Eur J Immunol. 2004; 34(3):623-630. (Biology). View Reference
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Dzionek A et al. BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood.. J Immunol. 2000; 165(11):6037-6046. (Biology). View Reference
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Mizui M, Kumanogoh A, Kikutani H. Immune semaphorins: novel features of neural guidance molecules.. J Clin Immunol. 2009; 29(1):1-11. (Biology). View Reference
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Pan Q et al. Neuropilin-1 binds to VEGF121 and regulates endothelial cell migration and sprouting.. J Biol Chem. 2007; 282(33):24049-24056. (Biology). View Reference
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Renand A, Milpied P, Rossignol J, et al. Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.. PLoS ONE. 2013; 8(12):e85589. (Biology). View Reference
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Soker S, Takashima S, Miao HQ, Neufeld G, Klagsbrun M. Neuropilin-1 is expressed by endothelial and tumor cells as an isoform-specific receptor for vascular endothelial growth factor.. Cell. 1998; 92(6):735-45. (Biology). View Reference
565954 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.